Application Data
(Figure 1. U2OS cells before and after drug treatment, imaging after incubation with Fluo-4 fluorescent probe (fluorescence microscope FITC filter))
ca2+ fluorescent (fluo-3) Probe
ca2+ fluorescent probe (fluo-3)
Synonyms
ca2+ fluorescent (fluo-3); N/A; ca2+ fluorescent probe (fluo-3); ca2+ fluorescent (fluo-3) probe
Application Data
(Figure 1. U2OS cells before and after drug treatment, imaging after incubation with Fluo-4 fluorescent probe (fluorescence microscope FITC filter))
Application Data
(Figure 1. U2OS cells before and after drug treatment, imaging after incubation with Fluo-4 fluorescent probe (fluorescence microscope FITC filter))
Application Data
(The Fluo-series shows enhanced fluorescence upon binding to Ca2+, unlike the UV-excited probes fura-2 and indo-1, and does not undergo spectral drift. The fluorescence optical density was enhanced at least 100-fold after binding to Ca 2 +. The spectral properties of Fluo-2, Fluo-3, and Fluo-4 are shown in following Table.)
Application Data
(The Fluo-series shows enhanced fluorescence upon binding to Ca2+, unlike the UV-excited probes fura-2 and indo-1, and does not undergo spectral drift. The fluorescence optical density was enhanced at least 100-fold after binding to Ca 2 +. The spectral properties of Fluo-2, Fluo-3, and Fluo-4 are shown in following Table.)
Related Product Information for ca2+ fluorescent (fluo-3) probe
This probe is suitable for detecting the intracellular calcium ion concentration (Ca2+Flow) change, is also often used in 96 or 384 wells GPCR high throughput drug screening.
Calcium ion Ca2+ indicated fluorescent probe Fluo series with Ca2+ in combination with the fluorescence enhancement, it is mainly used to detect all aspects of calcium ion signaling pathway, namely calcium flow, such as pharmacological screening at the cellular level, second messenger, neurotransmitter, photoactivation of chelator, etc.
Fluo-2 was one of the first Fluo series of calcium ion indicators invented by Dr. Roger Tsien. Fluo-2 is brighter in cells than Fluo-3 and Fluo-4, mainly probably due to the higher capacity of most cells to load Fluo-2 than Fluo-3 and Fluo-4. Therefore, Fluo-2 is often used for intracellular calcium ion imaging. Fluo-3 is often used on flow cytometry analysis because it can be excited by an argon ion laser 488nmand after binding with Ca2+, a very bright fluorescent signal is emitted.
Fluo-4 is an analogue of Fluo-3 replacing chlorine atoms with two fluorine atoms. This small structural change enhances the fluorescence signal of Fluo-4 probe at 488nm excitation light. The signal is stable and can be applied to confocal microscopy, flow cytometer, fluorescence luminometer / plate reader.
Calcium ion Ca2+ indicated fluorescent probe Fluo series with Ca2+ in combination with the fluorescence enhancement, it is mainly used to detect all aspects of calcium ion signaling pathway, namely calcium flow, such as pharmacological screening at the cellular level, second messenger, neurotransmitter, photoactivation of chelator, etc.
Fluo-2 was one of the first Fluo series of calcium ion indicators invented by Dr. Roger Tsien. Fluo-2 is brighter in cells than Fluo-3 and Fluo-4, mainly probably due to the higher capacity of most cells to load Fluo-2 than Fluo-3 and Fluo-4. Therefore, Fluo-2 is often used for intracellular calcium ion imaging. Fluo-3 is often used on flow cytometry analysis because it can be excited by an argon ion laser 488nmand after binding with Ca2+, a very bright fluorescent signal is emitted.
Fluo-4 is an analogue of Fluo-3 replacing chlorine atoms with two fluorine atoms. This small structural change enhances the fluorescence signal of Fluo-4 probe at 488nm excitation light. The signal is stable and can be applied to confocal microscopy, flow cytometer, fluorescence luminometer / plate reader.
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Product Notes
The ca2+ fluorescent (fluo-3) (Catalog #AAA269816) is a Probe and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ca2+ fluorescent (fluo-3), Probe" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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