Standard Curve (Sample)
Human Pan Trypsin ELISA Kit | PRSS1/2/3 elisa kit
Human Pan Trypsin PicoKine ELISA Kit
Gene Names
PRSS1; TRP1; TRY1; TRY4; TRYP1
Reactivity
Human
Synonyms
Pan Trypsin; N/A; Human Pan Trypsin PicoKine ELISA Kit; Trypsin-1; Beta-trypsin; Trypsin I; PRSS1; TRP1; TRY1; TRYP1; Trypsin-2; Anionic trypsinogen; Trypsin II; PRSS2; TRY2; TRYP2; Trypsin-3; Brain trypsinogen; Trypsin III; Trypsin IV; PRSS4; TRY3; TRY4; P07477; P07478; P35030; Trypsin-1/2/3; protease, serine 1/2/3; PRSS1/2/3 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human Pan Trypsin
Sequence Length
247
Samples
Cell culture supernatants, serum and plasma (heparin)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PRSS1/2/3 elisa kit
Background: Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.
Principle of the Assay: The ?Picokine™ Human Pan Trypsin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human Pan Trypsin with a 96-well strip plate that is pre-coated with antibody specific for Pan Trypsin. The detection antibody is a biotinylated antibody specific for Pan Trypsin. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human Pan Trypsin protein as standards. To measure Human Pan Trypsin, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human Pan Trypsin in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human Pan Trypsin in the sample.
Principle of the Assay: The ?Picokine™ Human Pan Trypsin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human Pan Trypsin with a 96-well strip plate that is pre-coated with antibody specific for Pan Trypsin. The detection antibody is a biotinylated antibody specific for Pan Trypsin. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human Pan Trypsin protein as standards. To measure Human Pan Trypsin, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human Pan Trypsin in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human Pan Trypsin in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,558 Da
NCBI Official Full Name
trypsin-1 preproprotein
NCBI Official Synonym Full Names
protease, serine 1
NCBI Official Symbol
PRSS1
NCBI Official Synonym Symbols
TRP1; TRY1; TRY4; TRYP1
NCBI Protein Information
trypsin-1
UniProt Protein Name
Trypsin-1
UniProt Gene Name
PRSS1
UniProt Synonym Gene Names
TRP1; TRY1; TRYP1
UniProt Entry Name
TRY1_HUMAN
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Product Notes
The Human PRSS1/2/3 prss1 (Catalog #AAA46699) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46699 ELISA Kit recognizes Human PRSS1/2/3. It is sometimes possible for the material contained within the vial of "Pan Trypsin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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