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Standard Curve (Sample)

Porcine P selectin ELISA Kit | PS elisa kit

Porcine P selectin ELISA Kit

Gene Names
SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
Reactivity
Porcine
Synonyms
P selectin; Porcine P selectin ELISA Kit; PS elisa kit
Ordering
For Research Use Only!
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of SELP. No significant cross-reactivity or interference between SELP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SELP and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Standard Curve (Sample)
Related Product Information for PS elisa kit
Principle of the Assay: SELP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SELP antibody and an SELP-HRP conjugate. The assay sample and buffer are incubated together with SELP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SELP concentration since SELP from samples and SELP-HRP conjugate compete for the anti-SELP antibody binding site. Since the number of sites is limited, as more sites are occupied by SELP from the sample, fewer sites are left to bind SELP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SELP concentration in each sample is interpolated from this standard curve.

Intended Uses: This SELP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SELP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for PS elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,834 Da
NCBI Official Full Name
P-selectin
NCBI Official Synonym Full Names
selectin P (granule membrane protein 140kDa, antigen CD62)
NCBI Official Symbol
SELP
NCBI Official Synonym Symbols
CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
NCBI Protein Information
P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
UniProt Protein Name
P-selectin
UniProt Gene Name
SELP
UniProt Synonym Gene Names
GMRP; GRMP; GMP-140; LECAM3; PADGEM
UniProt Entry Name
LYAM3_HUMAN

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Product Notes

The Porcine PS selp (Catalog #AAA16718) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16718 ELISA Kit recognizes Porcine PS. It is sometimes possible for the material contained within the vial of "P selectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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