Application Data
(Figure 2. Pseudoviral Particle (PP) Infection Assays (Ebola pseudoviral particles on HEK293 cells) (Luminescence plate reader: BioTek Synergy 2, gain 255)Legends: (-): lentivirus control (w/o envelope protein) Su2000: Ebola pseudovirus particles)
Sudan Ebolavirus 2000 Pseudovirus
Ebola Pseudoviral Particles, Sudan Ebolavirus 2000
Synonyms
Sudan Ebolavirus 2000; N/A; Ebola Pseudoviral Particles, Sudan Ebolavirus 2000; Sudan Ebolavirus 2000 pseudovirus
Form/Format
1 ml; OR 5 ml, for one multi-well plate. PP per mL > 1.0E+07
Features
Robust: Excellent signal to noise (basal) ratio
Easy to use: Amenable to HTS format (96-well, 384-well and 1536-well format)
Easy to use: Amenable to HTS format (96-well, 384-well and 1536-well format)
Preparation and Storage
Upon receiving it, store at -70 oC right away. Thaw* before immediate use. *Note: read the instruction for thawing in the following protocol carefully. Do not aliquot and refreeze. Shelf Life: Six months from the date of shipping when stored at -70 oC
Application Data
(Figure 2. Pseudoviral Particle (PP) Infection Assays (Ebola pseudoviral particles on HEK293 cells) (Luminescence plate reader: BioTek Synergy 2, gain 255)Legends: (-): lentivirus control (w/o envelope protein) Su2000: Ebola pseudovirus particles)
Application Data
(Figure 2. Pseudoviral Particle (PP) Infection Assays (Ebola pseudoviral particles on HEK293 cells) (Luminescence plate reader: BioTek Synergy 2, gain 255)Legends: (-): lentivirus control (w/o envelope protein) Su2000: Ebola pseudovirus particles)
Application Data
(Figure 1. Illustration of the replication-deficient lentivirus particle pseudotyped with Ebola virus surface Glycoprotein (GP).)
Application Data
(Figure 1. Illustration of the replication-deficient lentivirus particle pseudotyped with Ebola virus surface Glycoprotein (GP).)
Related Product Information for Sudan Ebolavirus 2000 pseudovirus
Ebola virus enters a cell by utilizing its surface glycoprotein (GP), which acts as a key to binding to specific receptors on the host cell membrane. This Ebola Pseudovirus is replication-deficient Lentivirus (LV) pseudotyped with the surface glycoprotein of Sudan ebolavirus (H.sapiens/Uganda/Gulu/2000)(GenBank: AY316199). The PseudoViral Particles contain the ORF for firefly luciferase as a reporter. These allow to establish an ebolavirus cell entry assay mediated by the Ebola virus glycoprotein which can be conveniently quantified via measuring luciferase activity in the cells. This pseudovirus assay isolates the Ebola viral entry from other steps of the viral infection cycle.
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Product Notes
The Sudan Ebolavirus 2000 (Catalog #AAA62286) is a Pseudovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Sudan Ebolavirus 2000, Pseudovirus" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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