Human Prostaglandin D synthase ELISA Kit | PTGDS elisa kit

Human Prostaglandin D synthase ELISA Kit

Cat. Num. AAA85538

• Gene Names: PTGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• Reactivity: Human
• Synonyms: Prostaglandin D synthase; N/A; Human Prostaglandin D synthase ELISA Kit; Human Prostaglandin D synthase (PTGDS) ELISA Kit; Prostaglandin D synthase (Human); PTGDS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGDS. No significant cross-reactivity or interference between PGDS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGDS and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for PTGDS elisa kit

Principle of the Assay: PGDS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PGDS antibody and an PGDS-HRP conjugate. The assay sample and buffer are incubated together with PGDS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGDS concentration since PGDS from samples and PGDS -HRP conjugate compete for the anti-PGDS antibody binding site. Since the number of sites is limited, as more sites are occupied by PGDS from the sample, fewer sites are left to bind PGDS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGDS concentration in each sample is interpolated from this standard curve.

Intended Uses: This PGDS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGDS. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for PTGDS elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 111080340
• NCBI GeneID: 5730
• UniProt Accession #: P41222; Q5SQ10; Q7M4P3; Q9UC22; Q9UCC9; Q9UCD9; B2R727
• Molecular Weight: 21,029 Da
• NCBI Official Full Name: prostaglandin D synthase
• NCBI Official Synonym Full Names: prostaglandin D2 synthase 21kDa (brain)
• NCBI Official Symbol: PTGDS
• NCBI Official Synonym Symbols: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• NCBI Protein Information: prostaglandin-H2 D-isomerase; cerebrin-28; PGD2 synthase; beta-trace protein; prostaglandin D synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; lipocalin-type prostaglandin-D synthase
• UniProt Protein Name: Prostaglandin-H2 D-isomerase
• UniProt Gene Name: PTGDS
• UniProt Synonym Gene Names: PDS; PGD2 synthase; PGDS; PGDS2
• UniProt Entry Name: PTGDS_HUMAN