Parathyroid Hormone Recombinant Protein | PTH recombinant protein

Human Parathyroid Hormone Recombinant

Cat. Num. AAA76063

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Parathyroid Hormone; N/A; Human Parathyroid Hormone Recombinant; pth; Parathyroid; PTH recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant PTH-84 was lyophilized from a 0.2 um filtered PB solution.
• Sequence: MIPAKDMAKV MIVMLAICFL TKSDGKSVKK RSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
• Sequence Length: 115

• Domain: PTH
• Host Note: Optimized DNA sequence encoding Human PTH mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human PTH, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 9 kDa. Recombinant Human PTH-84 is a disulfide-linked homodimeric protein consisting of 84 amino acid residue subunits, and migrates as an approximately 9 kDa under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for PTH recombinant protein

Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption; however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein α subunits.Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.

Product Categories/Family for PTH recombinant protein

Cytokines

References

• endogenous parathyroid hormone-related protein regulates the expression of PTH type 1 receptor and proliferation of vascular smooth muscle cells .Mol. Endocrinol., Oct 2009; 23: 1681 - 1690.
• PTH-induced internalization of apical membrane napi2a: role of actin and myosin vi .Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00260.2009.
• motoring down the microvilli. editorial focus on "PTH-induced internalization of apical membrane napi2a: role of actin and myosin vi" .Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00423.2009.
• simultaneous control of PTH and caxp is sustained over three years of treatment with cinacalcet hcl .Clin. J. Am. Soc. Nephrol., Sep 2009; 4: 1465 - 1476.
• human PTH gene regulation in vivo using transgenic mice .Am J Physiol Renal Physiol, Sep 2009; 297: F713 - F719.