SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPRB recombinant protein
PTPRB, Active
Applications
Western Blot
Synonyms
PTPRB; N/A; PTPRB, Active; PTPB; HPTPB; VEPTP; HPTP-BETA; R-PTP-BETA; PTPRB recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 20mM MOPS, pH7.5, 300mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for PTPRB recombinant protein
WB (Western Blot), Phosphatase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PTPRB recombinant protein
Recombinant human PTPRB (1552-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_002837.
PTPRB contains multiple repeated fibronectin type-lll domains extracellularly, a single transmembrane segment and only one protein tyrosine phosphatase (PTP) domain intracellularly, and thus belongs to receptor type PTP family (1). PTPRB negatively regulates angiogenesis and blood vessel remodeling via interacting with vascular growth factor tyrosine kinases such as TIE2, CDH5, and KDR (2). PTPRB has been reported to be one of the recurrent mutations in angiosarcoma tumors (3).
PTPRB contains multiple repeated fibronectin type-lll domains extracellularly, a single transmembrane segment and only one protein tyrosine phosphatase (PTP) domain intracellularly, and thus belongs to receptor type PTP family (1). PTPRB negatively regulates angiogenesis and blood vessel remodeling via interacting with vascular growth factor tyrosine kinases such as TIE2, CDH5, and KDR (2). PTPRB has been reported to be one of the recurrent mutations in angiosarcoma tumors (3).
Product Categories/Family for PTPRB recombinant protein
References
1. Crossland S. et al: Molecular cloning and characterization of PTP pi, a novel receptor-like protein-tyrosine phosphatase. Biochem J. 1996 Oct 1;319 (Pt 1):249-54.
2. Mantilidewi KI. et al: Shear stress-induced redistribution of vascular endothelial-protein-tyrosine phosphatase (VE-PTP) in endothelial cells and its role in cell elongation. J Biol Chem. 2014 Mar 7;289(10):6451-61.
3. Behjati S, et al: Recurrent PTPRB and PLCG1 mutations in angiosarcoma. Nat Genet. 2014 Apr;46(4):376-379.
2. Mantilidewi KI. et al: Shear stress-induced redistribution of vascular endothelial-protein-tyrosine phosphatase (VE-PTP) in endothelial cells and its role in cell elongation. J Biol Chem. 2014 Mar 7;289(10):6451-61.
3. Behjati S, et al: Recurrent PTPRB and PLCG1 mutations in angiosarcoma. Nat Genet. 2014 Apr;46(4):376-379.
NCBI and Uniprot Product Information
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Product Notes
The PTPRB (Catalog #AAA73841) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PTPRB can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Phosphatase Assay. Researchers should empirically determine the suitability of the PTPRB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPRB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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