Mouse PYD and CARD domain containing ELISA Kit | PYCARD elisa kit

Mouse Apoptosis-associated speck-like protein containing a CARD, PYCARD ELISA Kit

Cat. Num. AAA112654

• Gene Names: Pycard; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
• Reactivity: Mouse
• Synonyms: PYD and CARD domain containing; N/A; Mouse Apoptosis-associated speck-like protein containing a CARD, PYCARD ELISA Kit; Mouse Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA kit; ASC; CARD5; MGC10332; TMS; TMS-1; TMS1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1; PYCARD elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PYCARD. No significant cross-reactivity or interference between mouse PYCARD and analogues was observed.
• Sequence Length: 193
• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 7.81 pg/ml -500 pg/ml.
• Sensitivity: The minimum detectable dose of mouse PYCARD is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for PYCARD elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PYCARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYCARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCARD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCARD bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 165377185
• NCBI GeneID: 66824
• NCBI Accession #: NP_075747.3
• NCBI GenBank Nucleotide #: NM_023258.4
• UniProt Accession #: Q9EPB4; Q9D2W9
• Molecular Weight: 21,459 Da
• NCBI Official Full Name: apoptosis-associated speck-like protein containing a CARD
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: Pycard
• NCBI Official Synonym Symbols: Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD; PYD and CARD domain-containing protein
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• UniProt Gene Name: Pycard
• UniProt Synonym Gene Names: Asc; mASC
• UniProt Entry Name: ASC_MOUSE