Glycogen Phosphorylase, Muscle (PYGM) Recombinant Protein | PYGM recombinant protein

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

Cat. Num. AAA134557

• Gene Names: Pygm; PG; AI115133
• Applications: Immunoprecipitation, Immunoprecipitation, ELISA, Western Blot, SDS-Page
• Purity: >98%
• Synonyms: Glycogen Phosphorylase, Muscle (PYGM); N/A; Recombinant Glycogen Phosphorylase, Muscle (PYGM); PYGM recombinant protein

• Host: E Coli
• Purity/Purification: >98%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RKQISVRGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYG
• Sequence Length: 842

• Applicable Applications for PYGM recombinant protein: IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg11~Gly187 with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted isoelectric point: 6.0
• Predicted Molecular Mass: 21.7kDa
• Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Tissue Specificity: Skeletal Muscle, Brain, Heart, Kidney.
• Traits: Freeze-dried powder
• Note: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affect the charge of the protein.; 3. Post-translational modification:Phosphorylation, glycosylation, methylation etc.; 4. Post-translation clevage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Application Data

SDS-PAGE

Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 6755256
• NCBI GeneID: 19309
• NCBI Accession #: NP_035354.1
• NCBI GenBank Nucleotide #: NM_011224.1
• UniProt Accession #: Q9WUB3
• Molecular Weight: Predicted Molecular Weight: 22.0kDa; Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: glycogen phosphorylase, muscle form
• NCBI Official Synonym Full Names: muscle glycogen phosphorylase
• NCBI Official Symbol: Pygm
• NCBI Official Synonym Symbols: PG; AI115133
• NCBI Protein Information: glycogen phosphorylase, muscle form; myophosphorylase
• UniProt Protein Name: Glycogen phosphorylase, muscle form
• UniProt Gene Name: Pygm
• UniProt Entry Name: PYGM_MOUSE