Rabbit H3K27me2 Antibody | anti-H3K27me2 antibody

Histone H3K27me2 Polyclonal Antibody

Cat. Num. AAA10663

• Gene Names: HIST3H3; H3t; H3.4; H3/g; H3FT
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Dot Blot, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: H3K27me2; N/A; Histone H3K27me2 Polyclonal Antibody; H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; DiMethyl-Histone H3-K27; anti-H3K27me2 antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Sequence Length: 136

• Applicable Applications for anti-H3K27me2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Dot Blot (DB), ELISA (E), ChIP, ChIPseq
• Application Notes: WB 1:500 - 1:1000; DB 1:500 - 1:1000; IHC-P 1:50 - 1:200; IF/ICC 1:50 - 1:200; ELISA: Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.; ChIP: 5µg antibody for 5µg-10µg of Chromatin; ChIP-seq 1:20 - 1:100
• Cross-Reactivity: Human, Mouse, Rat (Wide Range Predicted)
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
• Preparation and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles

IF (Immunoflouorescence)

(Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

DB (Dot Blot)

(Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (AAA10663) at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of various lysates, using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25µg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

Application Data

(Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethylHistone H3-K27 antibody (AAA10663) and rabbit igG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IF (Immunofluorescence)

(Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody (AAA10663).Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K27 antibody (AAA10663) atdilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K27 antibody (AAA10663) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody(AAA10663) atdilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human liver cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

DB (Dot Blot)

(Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-H3K27me2 antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product Categories/Family for anti-H3K27me2 antibody

Methylation

NCBI and Uniprot Product Information

• NCBI GI #: 18202512
• NCBI GeneID: 8290
• UniProt Accession #: Q16695; Q6FGU4; B2R5K3
• Molecular Weight: Observed MW: 17kDa; Calculated MW: 16kDa
• NCBI Official Full Name: Histone H3.1t
• NCBI Official Synonym Full Names: histone cluster 3, H3
• NCBI Official Symbol: HIST3H3
• NCBI Official Synonym Symbols: H3t; H3.4; H3/g; H3FT
• NCBI Protein Information: histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
• UniProt Protein Name: Histone H3.1t
• UniProt Gene Name: HIST3H3
• UniProt Synonym Gene Names: H3FT; H3/t; H3t
• UniProt Entry Name: H31T_HUMAN