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IF (Immunoflouorescence)
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product-image-AAA10663_IF20.jpg IF (Immunoflouorescence) (Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

Rabbit H3K27me2 Antibody | anti-H3K27me2 antibody

Histone H3K27me2 Polyclonal Antibody

Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Dot Blot, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purification
Synonyms
H3K27me2; N/A; Histone H3K27me2 Polyclonal Antibody; H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; DiMethyl-Histone H3-K27; anti-H3K27me2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Sequence Length
136
Applicable Applications for anti-H3K27me2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Dot Blot (DB), ELISA (E), ChIP, ChIPseq
Application Notes
WB 1:500 - 1:1000
DB 1:500 - 1:1000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
ELISA: Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP: 5µg antibody for 5µg-10µg of Chromatin
ChIP-seq 1:20 - 1:100
Cross-Reactivity
Human, Mouse, Rat (Wide Range Predicted)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
Preparation and Storage
Store at -20°C. Avoid repeated freeze/thaw cycles

IF (Immunoflouorescence)

(Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

product-image-AAA10663_IF20.jpg IF (Immunoflouorescence) (Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

product-image-AAA10663_IHC19.jpg IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)

product-image-AAA10663_IHC18.jpg IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

product-image-AAA10663_IHC17.jpg IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

DB (Dot Blot)

(Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (AAA10663) at 1:1000 dilution.)

product-image-AAA10663_DB16.jpg DB (Dot Blot) (Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (AAA10663) at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of various lysates, using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25µg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

product-image-AAA10663_WB15.jpg WB (Western Blot) (Western blot analysis of various lysates, using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25µg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

Application Data

(Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethylHistone H3-K27 antibody (AAA10663) and rabbit igG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

product-image-AAA10663_APP14.jpg Application Data (Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethylHistone H3-K27 antibody (AAA10663) and rabbit igG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IF (Immunofluorescence)

(Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody (AAA10663).Blue: DAPI for nuclear staining.)

product-image-AAA10663_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody (AAA10663).Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K27 antibody (AAA10663) atdilution of 1:100 (40x lens).)

product-image-AAA10663_IHC12.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K27 antibody (AAA10663) atdilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K27 antibody (AAA10663) at dilution of 1:100 (40x lens).)

product-image-AAA10663_IHC11.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K27 antibody (AAA10663) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody(AAA10663) atdilution of 1:100 (40x lens).)

product-image-AAA10663_IHC10.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody(AAA10663) atdilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

product-image-AAA10663_IHC9.jpg IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

product-image-AAA10663_IHC8.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

product-image-AAA10663_IHC7.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human liver cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

product-image-AAA10663_IHC6.jpg IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

product-image-AAA10663_IHC5.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

product-image-AAA10663_IHC4.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

product-image-AAA10663_IHC3.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).)

DB (Dot Blot)

(Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody at 1:1000 dilution.)

product-image-AAA10663_DB2.jpg DB (Dot Blot) (Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

product-image-AAA10663_WB.jpg WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-H3K27me2 antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Product Categories/Family for anti-H3K27me2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 17kDa
Calculated MW: 16kDa
NCBI Official Full Name
Histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN

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Product Notes

The H3K27me2 hist3h3 (Catalog #AAA10663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's H3K27me2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Dot Blot (DB), ELISA (E), ChIP, ChIPseq. WB 1:500 - 1:1000 DB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA: Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. ChIP: 5µg antibody for 5µg-10µg of Chromatin ChIP-seq 1:20 - 1:100. Researchers should empirically determine the suitability of the H3K27me2 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3K27me2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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