FCM (Flow Cytometry)
(Flow cytometry: K562 cells were stained with Rabbit IgG isotype control(AAA28629, 10 ug/mL, blue line) followed by goat anti-Rabbit pAb APC(1:600 dilution) staining. Non-fluorescently stained K562 cells was used as blank control (red line).)
Rabbit IgG isotype control Antibody | anti-IgG antibody
Rabbit IgG isotype control
IF/ICC: 1:50-1:200
IP: 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
FC: 1:50-1:200
ChIP: 3ug antibody for 10ug-15ug of Chromatin
FCM (Flow Cytometry)
(Flow cytometry: K562 cells were stained with Rabbit IgG isotype control(AAA28629, 10 ug/mL, blue line) followed by goat anti-Rabbit pAb APC(1:600 dilution) staining. Non-fluorescently stained K562 cells was used as blank control (red line).)
FCM (Flow Cytometry)
(Flow cytometry: K562 cells were stained with Rabbit IgG isotype control(AAA28629, 10 ug/mL, blue line) followed by goat anti-Rabbit pAb APC(1:600 dilution) staining. Non-fluorescently stained K562 cells was used as blank control (red line).)
FCM (Flow Cytometry)
(Flow cytometry: Daudi cells were stained with Rabbit IgG isotype control(AAA28629, 10 ug/mL, blue line) followed by goat anti-Rabbit pAb APC(1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).)
FCM (Flow Cytometry)
(Flow cytometry: Daudi cells were stained with Rabbit IgG isotype control(AAA28629, 10 ug/mL, blue line) followed by goat anti-Rabbit pAb APC(1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 15 ug of cross-linked chromatin from HeLa cells transfected with a RXRB expression vector containing a single C-terminal DYKDDDDK-Tag, using 3 ug of Rabbit IgG isotype control (AAA28629) and RXRB Rabbit mAb (A25648). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 15 ug of cross-linked chromatin from HeLa cells transfected with a RXRB expression vector containing a single C-terminal DYKDDDDK-Tag, using 3 ug of Rabbit IgG isotype control (AAA28629) and RXRB Rabbit mAb (A25648). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.)
ICC (Immunocytochemistry)
(Confocal imaging of PC-12 cells using Rabbit IgG isotype control (AAA28629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of PC-12 cells using Rabbit IgG isotype control (AAA28629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of NIH/3T3 cells using Rabbit IgG isotype control (AAA28629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of NIH/3T3 cells using Rabbit IgG isotype control (AAA28629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using Rabbit IgG isotype control (AAA28629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using Rabbit IgG isotype control (AAA28629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat kidney using Rabbit IgG isotype control (AAA28629) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat kidney using Rabbit IgG isotype control (AAA28629) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Rabbit IgG isotype control (AAA28629) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Rabbit IgG isotype control (AAA28629) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rabbit IgG isotype control (AAA28629) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rabbit IgG isotype control (AAA28629) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
Similar Products
Product Notes
The IgG (Catalog #AAA28629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG isotype control can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Chromatin Immunoprecipitation (ChIP). IHC-P: 1:50-1:200 IF/ICC: 1:50-1:200 IP: 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells FC: 1:50-1:200 ChIP: 3ug antibody for 10ug-15ug of Chromatin. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG isotype control, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.