IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 ? overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Rabbit SFRP3 Antibody | anti-SFRP3 antibody
SFRP3 Antibody
Gene Names
FRZB; FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
SFRP3; N/A; SFRP3 Antibody; Secreted frizzled-related protein 3 (sFRP-3) (Frezzled) (Fritz) (Frizzled-related protein 1) (FrzB-1); anti-SFRP3 antibody
Host
Rabbit
Isotype
IgG`
Specificity
SFRP3 Polyclonal Antibody detects endogenous levels of protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
325
Applicable Applications for anti-SFRP3 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Synthesized peptide derived from human protein . at AA range: 150-230
Conjugation
FITC
Function
disease:Defects in FRZB are associated with female-specific osteoarthritis (OA) susceptibility [MIM:165720]. It is the most prevalent form of arthritis and a common cause of disability.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.,similarity:Belongs to the secreted frizzled-related protein (sFRP) family.,similarity:Contains 1 FZ (frizzled) domain.,similarity:Contains 1 NTR domain.
Subcellular Location
Secreted
Expression
Expressed primarily in the cartilaginous cores of the long bone during embryonic and fetal development and in the appendicular skeleton (6-13 weeks). At 13 weeks of gestation, transcripts were present in early chondroblasts of the tarsal bones of the foot, the carpal bones of the hands and the epiphysis of long bones. Highly expressed in placenta and heart, followed by brain, skeletal muscle, kidney and pancreas. Weakly expressed in lung and liver.
Preparation and Storage
Store at -15°C to -25°C/1 year (Do not lower than -25°C).
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 ? overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 ? overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
WB (Western Blot)
(Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night.)
WB (Western Blot)
(Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night.)
Related Product Information for anti-SFRP3 antibody
Background:The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed Band(KD):35kD
NCBI Official Full Name
secreted frizzled-related protein 3
NCBI Official Synonym Full Names
frizzled related protein
NCBI Official Symbol
FRZB
NCBI Official Synonym Symbols
FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
NCBI Protein Information
secreted frizzled-related protein 3
UniProt Protein Name
Secreted frizzled-related protein 3
UniProt Gene Name
FRZB
UniProt Synonym Gene Names
FIZ; FRE; FRP; FRZB1; SFRP3; sFRP-3
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Product Notes
The SFRP3 frzb (Catalog #AAA267983) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SFRP3 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SFRP3 frzb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRP3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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