Application Data
Rabbit S6K (pS434) Antibody | anti-S6K (pS434) antibody
Rabbit anti Phospho-p70S6K (pSer434)
Reactivity
Human, Rat, Mouse, Chicken, Bovine. Canine
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
S6K (pS434); N/A; Rabbit anti Phospho-p70S6K (pSer434); Rabbit anti-p70S6K (pS434); RPS6KB1; S6K kinase; Ribosomal protein S6K kinase; p70S6K; anti-S6K (pS434) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Bovine. Canine
Specificity
This antibody recognizes ~70 kDa of human p70S6K protein. It does not recognize non-phosphorylated peptides. This antibody also reacts with mouse and rat. The other species are not tested
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-S6K (pS434) antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Preparation
A synthetic peptide corresponding to the epitope -IRSPR- at a phosphorylation site at serine 434 of human p70S6K protein. This sequence is identical within human, rat, mouse, bovine, chicken and dog
Positive Control
Conditioned 3T3 cells
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Application Data
Application Data
Related Product Information for anti-S6K (pS434) antibody
S6 kinase, or p70 S6 Kinase, a mitogen activated Ser/Thr protein kinase, is required for cell growth and G1 cell cycle progression. P70 S6 Kinase and single 4E-BP are phosphorylated via the insulin and target of rapamycin (TOR) signaling pathway which is independent of PI3K and AKT activity. On the other hand, the oncogenic transformation by P3k and Akt is dependent on targets that control translation: transforming activity, which is correlated with phosphorylation and activation of S6K and eliminated by the mTOR inhibitor rapamycin. S6K phosphorylates the S6 protein of the 40S ribosomal subunit.
References
Mathieu Miron, et al. Signaling from Akt to FRAP/TOR Targets both 4E-BP and S6K in Drosophila melanogaster. (2003) Molecular and Cellular Biology, 23 (24), 9117-9126.
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Product Notes
The S6K (pS434) (Catalog #AAA71399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho-p70S6K (pSer434) reacts with Human, Rat, Mouse, Chicken, Bovine. Canine and may cross-react with other species as described in the data sheet. AAA Biotech's S6K (pS434) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the S6K (pS434) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S6K (pS434), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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