Rabbit MAFG Antibody | anti-MAFG antibody

MAFG Antibody (N-term)

Cat. Num. AAA289048

• Gene Names: MAFG; hMAF
• Reactivity: Human, mouse ; Predicted: Bovine, Chicken
• Applications: ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MAFG; N/A; MAFG Antibody (N-term); Transcription factor MafG; V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; hMAF; anti-MAFG antibody

• Host: Rabbit
• Reactivity: Human, mouse ; Predicted: Bovine, Chicken
• Isotype: Rabbit Ig
• Specificity: This MAFG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAFG.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1-30
• Sequence Length: 162

• Applicable Applications for anti-MAFG antibody: ELISA, WB (Western Blot)
• Calculated MW: 17850
• Function: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9421508, PubMed:11154691). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed:11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed:11154691). Activates globin gene expression when associated with NFE2L2 (PubMed:1115469.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(MAFG Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAFG antibody detected the MAFG protein (arrow).)

Related Product Information for anti-MAFG antibody

Globin gene expression is regulated through nuclear factor
erythroid-2 (NFE2) elements located in enhancer-like locus control
regions positioned many kb upstream of alpha- and beta-gene
clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2
DNA-binding activity consists of a heterodimer containing a
ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM
600197) and the tissue-restricted protein p45 NFE2 (MIM 601490).
Both subunits are members of the activator protein-1-like
superfamily of basic leucine zipper (bZIP) proteins (see MIM
165160).

Product Categories/Family for anti-MAFG antibody

Cell Biology; Metabolism

References

Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
Berg, D.T., et al. J. Biol. Chem. 282(51):36837-36844(2007)
Yamamoto, T., et al. Genes Cells 11(6):575-591(2006)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Tramier, M., et al. Biophys. J. 83(6):3570-3577(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 4505073
• NCBI GeneID: 4097
• NCBI Accession #: NP_002350.1; NP_116100.2
• NCBI GenBank Nucleotide #: NM_002359.3
• UniProt Accession #: O15525
• NCBI Official Full Name: transcription factor MafG
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G
• NCBI Official Symbol: MAFG
• NCBI Official Synonym Symbols: hMAF
• NCBI Protein Information: transcription factor MafG
• UniProt Protein Name: Transcription factor MafG
• UniProt Gene Name: MAFG
• UniProt Entry Name: MAFG_HUMAN