Application Data
(Image: Detection of autophagic LC3 in mouse ES cell lysate. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of LC3, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center)
Rabbit LC3 Antibody | anti-LC3 antibody
LC3, Affinity Purified Antibody, Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Xenopus
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified Antibody
Synonyms
LC3; N/A; LC3, Affinity Purified Antibody, Rabbit; anti-LC3 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Xenopus
Purity/Purification
Affinity Purified Antibody
Form/Format
Liquid. PBS, 30% glycerol and 0.02% Sodium Azide. 1.2 mg/ml.
Applicable Applications for anti-LC3 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Type Info
Rabbit IgG
Immunogen Sequence
A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121).
Preparation and Storage
Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C. Repeated freeze/thaw cycles compromise the integrity of the antiserum.
Application Data
(Image: Detection of autophagic LC3 in mouse ES cell lysate. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of LC3, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center)
Application Data
(Image: Detection of autophagic LC3 in mouse ES cell lysate. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of LC3, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center)
Application Data
(Image: LC3 staining of Human Cerebral Cortex)
Application Data
(Image: LC3 staining of Human Cerebral Cortex)
Related Product Information for anti-LC3 antibody
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Product Categories/Family for anti-LC3 antibody
Similar Products
Product Notes
The LC3 (Catalog #AAA76460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LC3, Affinity Purified Antibody, Rabbit reacts with Human, Rat, Mouse, Bovine, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's LC3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the LC3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LC3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.