Rabbit plasmin-antiplasmin complex, PAP ELISA Kit | PAP elisa kit
Rabbit plasmin-antiplasmin complex, PAP ELISA Kit
Reactivity
Rabbit
Synonyms
plasmin-antiplasmin complex, PAP; N/A; Rabbit plasmin-antiplasmin complex, PAP ELISA Kit; Rabbit plasmin-antiplasmin complex; PAP ELISA Kit; plasmin-antiplasmin complex; PAP; PAP elisa kit
Reactivity
Rabbit
Specificity
This assay has high sensitivity and excellent specificity for detection of human pAMPK. No significant cross-reactivity or interference between human pAMPK and analogues was observed.
Sequence Length
168
Samples
Serum, Plasma, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
1.56 pg/ml-100 pg/ml
Sensitivity
0.39 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for PAP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for pAMPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pAMPK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pAMPK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pAMPK bound in the initial step. The color development is stopped and the intensity of the color is measured.