WB (Western Blot)
(Western blot analysis of A549 cell lysate using NR1D1 antibody.)
Rabbit Rev-erbalpha Antibody | anti-hRev antibody
Rev-erbalpha Antibody
Gene Names
NR1D1; EAR1; hRev; THRA1; THRAL; ear-1; REVERBA; REVERBalpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
Rev-erbalpha; N/A; Rev-erbalpha Antibody; NR1D1; EAR1; THRA1; THRAL; ear-1; anti-hRev antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
614
Applicable Applications for anti-hRev antibody
WB (Western Blot)
Immunogen
Fusion protein of human NR1D1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of A549 cell lysate using NR1D1 antibody.)
WB (Western Blot)
(Western blot analysis of A549 cell lysate using NR1D1 antibody.)
Related Product Information for anti-hRev antibody
Reverse orientation c-erbA gene alpha (Rev-erbalpha, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbalpha is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbalpha potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbalpha regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3, 4, 5, 6, 7). Phosphorylation of Rev-erbalpha Ser55 and Ser59 by GSK3beta appears to stabilize Rev-erbalpha protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbalpha also regulates inflammation by targeting the NF-kappaB responsive genes IL-6 and COX-2 (9). Rev-erbalpha lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10).
Product Categories/Family for anti-hRev antibody
References
1. Harding, H.P. and Lazar, M.A. (1993) Mol. Cell Biol. 13, 3113-3121. 2. Chawla, A. and Lazar, M.A. (1993) J. Biol. Chem. 268, 16265-16269. 3. Torra, I.P. et al. (2000) Endocrinology 141, 3799-3806. 4. Preitner, N. et al. (2002) Cell 110, 251-260. 5. Vu-Dac, N. et al. (1998) J. Biol.Chem. 273, 25713-25720. 6. Coste, H. and Rodríguez, J.C. (2002) J. Biol. Chem. 277, 27120-27129. 7. Raspé, E. et al. (2002) J. Lipid Res. 43, 2172-2179. 8. Yin, L. et al. (2006) Science 311, 1002-1005. 9. Migita, H. et al. (2004) FEBS Lett. 561, 69-74. 10. Yin, L. and Lazar, M.A. (2005) Mol. Endocrinol. 19, 1452-1459.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67kDa
NCBI Official Full Name
nuclear receptor subfamily 1 group D member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 1 group D member 1
NCBI Official Symbol
NR1D1
NCBI Official Synonym Symbols
EAR1; hRev; THRA1; THRAL; ear-1; REVERBA; REVERBalpha
NCBI Protein Information
nuclear receptor subfamily 1 group D member 1
UniProt Protein Name
Nuclear receptor subfamily 1 group D member 1
UniProt Gene Name
NR1D1
UniProt Synonym Gene Names
EAR1; HREV; THRAL; EAR-1
Similar Products
Product Notes
The hRev nr1d1 (Catalog #AAA267173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rev-erbalpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rev-erbalpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the hRev nr1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rev-erbalpha, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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