Rabbit anti-Human Cycloxygenase-2 (COX-2) Antibody | anti-COX-2 antibody

Cycloxygenase-2 (COX-2)

Cat. Num. AAA215169

• Gene Names: PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cycloxygenase-2 (COX-2); N/A; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; Macrophage activation-associated marker protein P71/73; PES-2; PGG/HS; PGHS-2; Prostaglandin endoperoxide synthase 2; Prostaglandin endoperoxide synthase 2 (PTGS2); TIS10; anti-COX-2 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Clone Number: COX2/3232R
• Specificity: Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90% of human colorectal adenocarcinomas whereas levels of COX-1 are not changed.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 604

• Applicable Applications for anti-COX-2 antibody: IHC (Immunohistochemistry)
• Positive Control: Human colon or lung carcinoma tissue (IHC).
• Immunogen: Recombinant human COX2 protein fragment (around aa 442-572) (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 1q31.1
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Endometrium stained with COX-2 Recombinant Rabbit Monoclonal Antibody (COX2/3232R).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with COX-2 Recombinant Rabbit Monoclonal Antibody (COX2/3232R).)

References

Wang, D, et al. Oncogene. 2010 Feb 11; 29(6): 781-788.

NCBI and Uniprot Product Information

• NCBI GI #: 4506265
• NCBI GeneID: 5743
• NCBI Accession #: NP_000954.1
• NCBI GenBank Nucleotide #: NP_000954.1
• UniProt Accession #: P35354
• Molecular Weight: 70-72kDa
• NCBI Official Full Name: prostaglandin G/H synthase 2
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 2
• NCBI Official Symbol: PTGS2
• NCBI Official Synonym Symbols: COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• NCBI Protein Information: prostaglandin G/H synthase 2
• UniProt Protein Name: Prostaglandin G/H synthase 2
• UniProt Gene Name: PTGS2
• UniProt Synonym Gene Names: COX2; COX-2; PGH synthase 2; PGHS-2
• UniProt Entry Name: PGH2_HUMAN