DB (Dot Blot)
(Histone H3.3 acetyl-lysine 27 phospho Ser31 rAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3.3 acetyl-lysine 27 phospho Ser31. Peptides corresponding to regions around major sites of histone H3 phosphorylation (serine 10, serine 28 and serine 31) were spotted onto Nitrocellulose and probed with 91425 at 4 ug/mL. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Peptide H3.3 acetyl-lysine 27 phospho Ser31. Lane 2: Peptide H3.3 phospho Ser31 Lane 3: Peptide H3.3 acetyl-lysine 27 Lane 4: Unmodified H3.3 peptide. Lane 5: Peptide H3.1 acetyl-lysine 27 phospho Ser28 Lane 6: Peptide H3.1 acetyl-lysine 9 phospho Ser10)
Rabbit H3.3K27acS31ph Antibody | anti-H3.3K27acS31ph antibody
AbFlex H3.3K27acS31ph antibody (rAb)
Gene Names
H3F3A; H3F3; H3.3A
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
H3.3K27acS31ph; N/A; AbFlex H3.3K27acS31ph antibody (rAb); anti-H3.3K27acS31ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1 (varies by lot)
Applicable Applications for anti-H3.3K27acS31ph antibody
WB (Western Blot), DB (Dot Blot), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against a peptide containing acetyl-lysine 27 of human Histone H3 sequence phosphorylated at serine 31.
Abs Residue
Ser
Modification
Phosphorylated
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
DB (Dot Blot)
(Histone H3.3 acetyl-lysine 27 phospho Ser31 rAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3.3 acetyl-lysine 27 phospho Ser31. Peptides corresponding to regions around major sites of histone H3 phosphorylation (serine 10, serine 28 and serine 31) were spotted onto Nitrocellulose and probed with 91425 at 4 ug/mL. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Peptide H3.3 acetyl-lysine 27 phospho Ser31. Lane 2: Peptide H3.3 phospho Ser31 Lane 3: Peptide H3.3 acetyl-lysine 27 Lane 4: Unmodified H3.3 peptide. Lane 5: Peptide H3.1 acetyl-lysine 27 phospho Ser28 Lane 6: Peptide H3.1 acetyl-lysine 9 phospho Ser10)
DB (Dot Blot)
(Histone H3.3 acetyl-lysine 27 phospho Ser31 rAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3.3 acetyl-lysine 27 phospho Ser31. Peptides corresponding to regions around major sites of histone H3 phosphorylation (serine 10, serine 28 and serine 31) were spotted onto Nitrocellulose and probed with 91425 at 4 ug/mL. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Peptide H3.3 acetyl-lysine 27 phospho Ser31. Lane 2: Peptide H3.3 phospho Ser31 Lane 3: Peptide H3.3 acetyl-lysine 27 Lane 4: Unmodified H3.3 peptide. Lane 5: Peptide H3.1 acetyl-lysine 27 phospho Ser28 Lane 6: Peptide H3.1 acetyl-lysine 9 phospho Ser10)
WB (Western Blot)
(AbFlex Histone H3.3K27acS31ph rAb antibody tested by Western blot. Detection of H3.3K27acS31ph by Western blot analysis using 15 ug HeLa acid extracts and probed with antibody at 0.5 ug/ml. Lane 1: Acid extract of untreated HeLa cells. Lane 2: Acid extract of HeLa cells treated with sodium butyrate.)
WB (Western Blot)
(AbFlex Histone H3.3K27acS31ph rAb antibody tested by Western blot. Detection of H3.3K27acS31ph by Western blot analysis using 15 ug HeLa acid extracts and probed with antibody at 0.5 ug/ml. Lane 1: Acid extract of untreated HeLa cells. Lane 2: Acid extract of HeLa cells treated with sodium butyrate.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex H3.3K27acS31ph antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 17 million sequence tags were mapped to identify H3.3K27acS31ph binding sites. The image shows binding across a region of chromosome 5.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex H3.3K27acS31ph antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 17 million sequence tags were mapped to identify H3.3K27acS31ph binding sites. The image shows binding across a region of chromosome 5.)
Related Product Information for anti-H3.3K27acS31ph antibody
AbFlex H3.3K27acS31ph antibody (rAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3.3K27acS31ph antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody was directly labeled with biotin using the biotin ligase, BirA. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. One of the histone variants, H3.3, has a difference of 4 to 5 amino acid residues between the sequence variants of H3 (H3.1 and H3.2). TThe only contrasting residue is serine, contained at the N-terminus of the protein at position 31. Ser31 phosphorylation is known as a mitotic epigenetic modification. H3K27ac is also preferentially deposited in H3.3, therefore this H3.3K27acS31ph antibody was developed due to the high likelihood that H3.3 proteins contain the dual modification of both acetylation at lysine residue 27 and phosphorylation at serine residue 31.
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3.3K27acS31ph antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody was directly labeled with biotin using the biotin ligase, BirA. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. One of the histone variants, H3.3, has a difference of 4 to 5 amino acid residues between the sequence variants of H3 (H3.1 and H3.2). TThe only contrasting residue is serine, contained at the N-terminus of the protein at position 31. Ser31 phosphorylation is known as a mitotic epigenetic modification. H3K27ac is also preferentially deposited in H3.3, therefore this H3.3K27acS31ph antibody was developed due to the high likelihood that H3.3 proteins contain the dual modification of both acetylation at lysine residue 27 and phosphorylation at serine residue 31.
Product Categories/Family for anti-H3.3K27acS31ph antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.7kDa (159aa)
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone family member 3A
NCBI Official Symbol
H3F3A
NCBI Official Synonym Symbols
H3F3; H3.3A
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3F3A
UniProt Synonym Gene Names
H3.3A; H3F3
UniProt Entry Name
H33_HUMAN
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Product Notes
The H3.3K27acS31ph h3f3a (Catalog #AAA60421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AbFlex H3.3K27acS31ph antibody (rAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's H3.3K27acS31ph can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H3.3K27acS31ph h3f3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3.3K27acS31ph, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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