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Application Data
(AbFlex Histone H3K4me1 antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 4 million reads, and H3K4me1 data is shown for Chromosome 6.)
Rabbit Histone H3K4me1 Antibody | anti-H3K4me1 antibody
AbFlex Histone H3K4me1 antibody (rAb)
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Histone H3K4me1; N/A; AbFlex Histone H3K4me1 antibody (rAb); anti-H3K4me1 antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1 (varies by lot)
Applicable Applications for anti-H3K4me1 antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against a peptide containing mono-methyl lysine 4 of Histone H3.
Abs Residue
Lys
Modification
Methylated
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Application Data
(AbFlex Histone H3K4me1 antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 4 million reads, and H3K4me1 data is shown for Chromosome 6.)
Application Data
(AbFlex Histone H3K4me1 antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 4 million reads, and H3K4me1 data is shown for Chromosome 6.)
Application Data
(AbFlex Histone H3K4me1 antibody (rAb) specificity tested by Peptide Array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. AbFlex Histone H3K4me1 antibody (rAb) was applied to MODified Histone Peptide Array at a dilution of 1:20000. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted.)
Application Data
(AbFlex Histone H3K4me1 antibody (rAb) specificity tested by Peptide Array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. AbFlex Histone H3K4me1 antibody (rAb) was applied to MODified Histone Peptide Array at a dilution of 1:20000. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted.)
WB (Western Blot)
(AbFlex Histone H3K4me1 rAb antibody tested by Western blot. Detection of H3K4me1 by Western blot analysis using 20 ug HeLa nuclear extract and probed with antibody at 0.5 ug/ml.)
WB (Western Blot)
(AbFlex Histone H3K4me1 rAb antibody tested by Western blot. Detection of H3K4me1 by Western blot analysis using 20 ug HeLa nuclear extract and probed with antibody at 0.5 ug/ml.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex Histone H3K4me1 rAb antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 36 million sequence tags were mapped to identify H3K4me1 binding sites. The image shows binding across a region of chromosome 8.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex Histone H3K4me1 rAb antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 36 million sequence tags were mapped to identify H3K4me1 binding sites. The image shows binding across a region of chromosome 8.)
Related Product Information for anti-H3K4me1 antibody
AbFlex Histone H3K4me1 antibody (rAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, CUT&Tag and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K4me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K4me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Product Categories/Family for anti-H3K4me1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
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Product Notes
The H3K4me1 hist1h3a (Catalog #AAA60420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Histone H3K4me1 antibody (rAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K4me1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H3K4me1 hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K4me1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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