FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-p21 antibody Y13-238 (AAA72254). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of Y13-238 (AAA72254-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Human, Mouse p21 Antibody | anti-p21 antibody
Anti-p21 [Y13-238], Rabbit IgG
Gene Names
HRAS; CTLO; KRAS; HAMSV; HRAS1; KRAS2; RASH1; RASK2; Ki-Ras; p21ras; C-H-RAS; c-K-ras; H-RASIDX; c-Ki-ras; C-BAS/HAS; C-HA-RAS1
Reactivity
Human, Mouse
Applications
Immunoprecipitation
Purity
Protein A affinity purified
Synonyms
p21; N/A; Anti-p21 [Y13-238], Rabbit IgG; Anti-p21 [Y13-238]; Ras; p21ras; anti-p21 antibody
Host
Rabbit
Reactivity
Human, Mouse
Isotype
Rabbit IgG, Lambda
Clone Number
Y13-238
Specificity
The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21?s but not v-K-ras or rodent c-K-ras p21?s. This antibody also recognizes human c-H-ras and c-K-ras p21?s, but not human c-N-ras.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-p21 antibody
IP (Immunoprecipitation)
Immunogen
Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-p21 antibody Y13-238 (AAA72254). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of Y13-238 (AAA72254-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-p21 antibody Y13-238 (AAA72254). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of Y13-238 (AAA72254-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-p21 (AAA72254) Y13-238 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of Y13-238 (AAA72254-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72254-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-p21 (AAA72254) Y13-238 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of Y13-238 (AAA72254-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72254-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-p21 antibody
Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti-ras non-neutralising scFv (Cochet 1998).
Product Categories/Family for anti-p21 antibody
References
Furth et al. Monoclonal Antibodies to the p21 Products of the Transforming Gene of Harvey Murine Sarcoma Virus and of the Cellular ras Gene Family JOURNAL OF VIROLOGY, July 1982, p. 294-304 PMID:6287003
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,870 Da
NCBI Official Full Name
p21 protein, partial
NCBI Official Synonym Full Names
HRas proto-oncogene, GTPase
NCBI Official Symbol
HRAS
NCBI Official Synonym Symbols
CTLO; KRAS; HAMSV; HRAS1; KRAS2; RASH1; RASK2; Ki-Ras; p21ras; C-H-RAS; c-K-ras; H-RASIDX; c-Ki-ras; C-BAS/HAS; C-HA-RAS1
NCBI Protein Information
GTPase HRas
UniProt Protein Name
P21 protein
UniProt Gene Name
HRAS
UniProt Entry Name
P78460_HUMAN
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Product Notes
The p21 hras (Catalog #AAA72254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p21 [Y13-238], Rabbit IgG reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p21 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the p21 hras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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