WB (Western Blot)
(Western blot analysis of extracts of various lysates, using Acetyl-alpha Tubulin -K40 Rabbit pAb (AAA282423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.)
Rabbit Acetyl-alpha Tubulin -K40 Antibody | anti-TUBA1A antibody
Acetyl-alpha Tubulin -K40 Rabbit pAb
Gene Names
TUBA1A; LIS3; TUBA3; B-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
Acetyl-alpha Tubulin -K40; N/A; Acetyl-alpha Tubulin -K40 Rabbit pAb; LIS3; TUBA3; B-ALPHA-1; anti-TUBA1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-TUBA1A antibody
WB (Western Blot)
Positive Samples
HeLa, Mouse testis, Rat testis
Research Area
Signal Transduction, Cell Biology Developmental Biology, Cytoskeleton, Microtubules
Immunogen
A synthetic acetylated peptide around K40 of human alpha Tubulin.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack
Ship: Ice Pack
WB (Western Blot)
(Western blot analysis of extracts of various lysates, using Acetyl-alpha Tubulin -K40 Rabbit pAb (AAA282423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various lysates, using Acetyl-alpha Tubulin -K40 Rabbit pAb (AAA282423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa, using Acetyl-alpha Tubulin -K40 antibody (AAA282423) at 1:500 dilution.HeLa cells were treated by Sodium butyrate (5 mM) for 16 hours after serum-starvation 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa, using Acetyl-alpha Tubulin -K40 antibody (AAA282423) at 1:500 dilution.HeLa cells were treated by Sodium butyrate (5 mM) for 16 hours after serum-starvation 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
Related Product Information for anti-TUBA1A antibody
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Categories/Family for anti-TUBA1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,297 Da
NCBI Official Full Name
Tubulin alpha-1A chain
NCBI Official Synonym Full Names
tubulin, alpha 1a
NCBI Official Symbol
TUBA1A
NCBI Official Synonym Symbols
LIS3; TUBA3; B-ALPHA-1
NCBI Protein Information
tubulin alpha-1A chain; hum-a-tub1; hum-a-tub2; tubulin B-alpha-1; tubulin alpha-3 chain; tubulin, alpha, brain-specific
UniProt Protein Name
Tubulin alpha-1A chain
UniProt Gene Name
TUBA1A
UniProt Synonym Gene Names
TUBA3
UniProt Entry Name
TBA1A_HUMAN
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Product Notes
The TUBA1A tuba1a (Catalog #AAA282423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetyl-alpha Tubulin -K40 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Acetyl-alpha Tubulin -K40 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the TUBA1A tuba1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetyl-alpha Tubulin -K40, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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