Rabbit c-Jun- S63 Antibody | anti-Jun antibody

Phospho-c-Jun- S63 Polyclonal Antibody

Cat. Num. AAA36753

• Gene Names: JUN; AP1; AP-1; c-Jun
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: c-Jun- S63; N/A; Phospho-c-Jun- S63 Polyclonal Antibody; Phospho-Jun-S63: AP1; AP-1; c-Jun; JUN; anti-Jun antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 331

• Applicable Applications for anti-Jun antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Route: Synthetic Peptide
• Immunogen: A phospho specific peptide corresponding to residues surrounding S63 of human c-Jun
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of MCF-7 cells using Phospho-Jun-S63 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of 293 cells, using Phospho-Jun-S63 antibody at 1:1000 dilution. 293 cells were treated by Anisomycin (25ug/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Related Product Information for anti-Jun antibody

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

Product Categories/Family for anti-Jun antibody

Phosphorylation

NCBI and Uniprot Product Information

• NCBI GI #: 135298
• NCBI GeneID: 3725
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 331
• NCBI Official Full Name: Transcription factor AP-1
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1; p39; jun oncogene; activator protein 1; proto-oncogene c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; v-jun sarcoma virus 17 oncogene homolog; v-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN