Application Data
Rabbit IRS-1 (pS312), Antibody | anti-IRS-1 (pS312), antibody
Rabbit anti Phospho- IRS-1 (pSer 312) antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Rat, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
IRS-1 (pS312),; N/A; Rabbit anti Phospho- IRS-1 (pSer 312) antibody; Rabbit anti-IRS-1 (p Ser312); Anti-phospho-IRS-1 (pSer312); Insulin-receptor substrate 1 (IRS-1).; anti-IRS-1 (pS312), antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Specificity
This antibody recognizes ~165 kDa of human IRS-1 (pSer312). It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-IRS-1 (pS312), antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Preparation
A synthetic peptide corresponding to the epitope ATSPA with a single phosphorylation site Ser 312 of human IRS-1.
Positive Control
CHO cell lysate
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Application Data
Application Data
Related Product Information for anti-IRS-1 (pS312), antibody
Insulin Receptor Substrate-1 (IRS-1), 165 kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser312 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Tyr612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKC, resulting in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
References
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
165 kDa
NCBI Official Full Name
Insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
NCBI Protein Information
insulin receptor substrate 1; IRS-1; OTTHUMP00000164234
UniProt Protein Name
Insulin receptor substrate 1
UniProt Gene Name
IRS1
UniProt Entry Name
IRS1_HUMAN
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Product Notes
The IRS-1 (pS312), irs1 (Catalog #AAA71386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- IRS-1 (pSer 312) antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRS-1 (pS312), can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IRS-1 (pS312), irs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRS-1 (pS312),, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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