Rabbit JAK2 (pY1007/Y1008) Antibody | anti-JAK2 (pY1007/Y1008) antibody

Rabbit anti Phospho- JAK2 (pTyr 1007/1008)

Cat. Num. AAA14087

• Gene Names: JAK2; JTK10
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Synonyms: JAK2 (pY1007/Y1008); N/A; Rabbit anti Phospho- JAK2 (pTyr 1007/1008); Rabbit anti-Phospho-JAK2; phosphospecific-Janus Kinase 2; anti-JAK2 (pY1007/Y1008) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Specificity: This antibody recognizes JAK2 with dual phosphorylated sites of Tyr1007/1008. It does not cross-react with nonphosphospecific peptide.
• Purity/Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

• Applicable Applications for anti-JAK2 (pY1007/Y1008) antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-10 ug/ml; ; Optimal dilutions should be determined by researchers for specific applications.
• Antigen Preparation: A synthetic peptide surrounding to the epitope -EYYK- with dual phosphorylation sites at Tyr1007 and Tyr1008 of JAK2 from human, mouse and rat origins.
• Positive Control: Kidney Tissue
• Cellular Location: Cell Membrane
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C - 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.

DB (Dot Blot)

(Dot Blot: 1 ug peptide was blot onto NC membraneA: JAK2 (pY1008, pY1009)B: JAK2 (Non phosphospecific)C: Non-related Phosphopeptide) were blotted at a 1:2000 dilution by:1: Rabbit anti-JAK2(pY1008/pY1009) (AAA14087)2: Rabbit anti-JAK2 (Nonphospho specific).)

WB (Western Blot)

(Western Blot: The tissue lysate derived from GH stimulated mouse liver was immunoblotted by Rabbit anti JAK2 (pY1007/pY1008) (AAA14087) antibody at 1:500.)

Related Product Information for anti-JAK2 (pY1007/Y1008) antibody

The Janus tyrosine kinase family is consists of four members, JAK1, JAK2, JAK3 and Tyk2. Upon the cytokine ligand binding to its the receptors, the dimerization of the receptors occurs followed by phosphorylation of the receptors. The phosphorylation of receptors recruites the tyrosine kinase physically associated to the dimerized receptors, and phosphorylate the kinase, thus activates the signal transducer and activator of thranscription (STAT1, 3, 5) and the other signal proteins.

References

Yogev,O., et al. SHP-2 regulates SOCS-1-mediated Janus kinase-2 ubiquitination/degradation downstream of the prolactin receptor. J. Biol. Chem. 278 (52), 52021-52031 (2003)

NCBI and Uniprot Product Information

• NCBI GI #: 4826776
• NCBI GeneID: 3717
• NCBI Accession #: NP_004963.1
• NCBI GenBank Nucleotide #: NM_004972.2
• UniProt Accession #: O60674; O14636; O75297; Q506Q0; Q52R86; Q8IXP2
• Molecular Weight: 120.0
• NCBI Official Full Name: tyrosine-protein kinase JAK2
• NCBI Official Synonym Full Names: Janus kinase 2
• NCBI Official Symbol: JAK2
• NCBI Official Synonym Symbols: JTK10
• NCBI Protein Information: tyrosine-protein kinase JAK2; JAK-2; OTTHUMP00000021024; Janus kinase 2 (a protein tyrosine kinase)
• UniProt Protein Name: Tyrosine-protein kinase JAK2
• UniProt Gene Name: JAK2
• UniProt Entry Name: JAK2_HUMAN