IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-LIMK2-T505 antibody.)
Rabbit LIMK2-T505 Antibody | anti-LIMK2 antibody
Phospho-LIMK2-T505 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
LIMK2-T505; N/A; Phospho-LIMK2-T505 Polyclonal Antibody; Phospho-LIMK2-T505: N/A; anti-LIMK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
638
Applicable Applications for anti-LIMK2 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2
Calculated Molecular Weight
70kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-LIMK2-T505 antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-LIMK2-T505 antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-LIMK2-T505 antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-LIMK2-T505 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using Phospho-LIMK2-T505 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using Phospho-LIMK2-T505 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Related Product Information for anti-LIMK2 antibody
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-LIMK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
638
NCBI Official Full Name
LIM domain kinase 2
NCBI Official Synonym Full Names
LIM domain kinase 2
NCBI Official Symbol
LIMK2
NCBI Protein Information
LIM domain kinase 2
UniProt Protein Name
LIM domain kinase 2
UniProt Gene Name
LIMK2
UniProt Synonym Gene Names
LIMK-2
UniProt Entry Name
LIMK2_HUMAN
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Product Notes
The LIMK2 limk2 (Catalog #AAA37370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-LIMK2-T505 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LIMK2-T505 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LIMK2 limk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMK2-T505, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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