Rabbit NOTCH3 Antibody | anti-NOTCH3 antibody

NOTCH3 Antibody

Cat. Num. AAA266858

• Gene Names: NOTCH3; IMF2; LMNS; CASIL; CADASIL; CADASIL1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: NOTCH3; N/A; NOTCH3 Antibody; CASIL; CADASIL; anti-NOTCH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 2321

• Applicable Applications for anti-NOTCH3 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide of human NOTCH3
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human colon cancer tissue using NOTCH3 antibody.)

Application Data

WB (Western Blot)

(Western blot analysis of extracts of human colon cancer tissue lysate, using NOTCH3 antibody.)

Related Product Information for anti-NOTCH3 antibody

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4). Notch3 is a member of the Notch family and is processed similar to Notch1 (5). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the Notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8). Recent studies indicate that Notch3 is overexpressed in many types of cancer (9-11).

Product Categories/Family for anti-NOTCH3 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. 2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. 3. Schroeter, E.H. et al. (1998) Nature 393, 382-6. 4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35. 5. Baron, M. (2003) Semin Cell Dev Biol 14, 113-9. 6. Kalimo, H. et al. (2002) Brain Pathol 12, 371-84. 7. Karlström, H. et al. (2002) Proc Natl Acad Sci U S A 99, 17119-24. 8. Monet, M. et al. (2007) Hum Mol Genet 16, 982-92. 9. Park, J.T. et al. (2006) Cancer Res 66, 6312-8. 10. Gramantieri, L. et al. (2007) Liver Int 27, 997-1007. 11. Yamaguchi, N. et al. (2008) Cancer Res 68, 1881-8.

NCBI and Uniprot Product Information

• NCBI GI #: 134244285
• NCBI GeneID: 4854
• NCBI Accession #: NP_000426.2
• NCBI GenBank Nucleotide #: NM_000435.2
• UniProt Accession #: Q9UM47; Q9UEB3; Q9UPL3; Q9Y6L8
• Molecular Weight: 244kDa
• NCBI Official Full Name: neurogenic locus notch homolog protein 3
• NCBI Official Synonym Full Names: notch 3
• NCBI Official Symbol: NOTCH3
• NCBI Official Synonym Symbols: IMF2; LMNS; CASIL; CADASIL; CADASIL1
• NCBI Protein Information: neurogenic locus notch homolog protein 3
• UniProt Protein Name: Neurogenic locus notch homolog protein 3
• UniProt Gene Name: NOTCH3
• UniProt Synonym Gene Names: Notch 3