WB (Western Blot)
(Western blot analysis of extracts of mouse lungcell lines, using PDGFRB antibody.)
Rabbit PDGFRB Antibody | anti-PDGFRB antibody
PDGFRB Antibody
Gene Names
PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
PDGFRB; N/A; PDGFRB Antibody; CD140B; JTK12; PDGF-R-beta; PDGFR; PDGFR1; anti-PDGFRB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
1106
Applicable Applications for anti-PDGFRB antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant protein of human PDGFRB
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of mouse lungcell lines, using PDGFRB antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse lungcell lines, using PDGFRB antibody.)
Related Product Information for anti-PDGFRB antibody
Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFRalpha) and PDGF receptor beta (PDGFRbeta). PDGFRalpha and PDGFRbeta share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRalpha homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRbeta homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor alpha/beta binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRalpha and PDGFRbeta can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLC gamma, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRbeta is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRbeta (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRbeta (7). Tyr740 is also required for PDGFRbeta-mediated PI3 kinase activation (8).
Product Categories/Family for anti-PDGFRB antibody
References
1. Deuel, T.F. et al. (1988) Biofactors 1, 213-217. 2. Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516. 3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507. 4. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45. 5. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38. 6. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272. 7. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272. 8. Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124kDa
NCBI Official Full Name
platelet-derived growth factor receptor beta isoform 1
NCBI Official Synonym Full Names
platelet derived growth factor receptor beta
NCBI Official Symbol
PDGFRB
NCBI Official Synonym Symbols
IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
NCBI Protein Information
platelet-derived growth factor receptor beta
UniProt Protein Name
Platelet-derived growth factor receptor beta
UniProt Gene Name
PDGFRB
UniProt Synonym Gene Names
PDGFR; PDGFR1; PDGF-R-beta; PDGFR-beta; PDGFR-1
Similar Products
Product Notes
The PDGFRB pdgfrb (Catalog #AAA266761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDGFRB Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFRB can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDGFRB pdgfrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFRB, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.