WB (Western Blot)
(Western blot analysis of A375 cell lysate using S100B antibody.)
Rabbit S100B Antibody | anti-S100B antibody
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
S100B; N/A; S100B Antibody; NEF; S100; S100beta; anti-S100B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
92
Applicable Applications for anti-S100B antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant protein of human S100B
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of A375 cell lysate using S100B antibody.)
WB (Western Blot)
(Western blot analysis of A375 cell lysate using S100B antibody.)
Related Product Information for anti-S100B antibody
Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4).Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo-or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4). S100B is abundantly expressed in astrocytes and is commonly used as an astrocytic marker in studies of the mammalian CNS. S100B is also expressed in immature and mature myelinating oligodendrocytes that are chondroitin sulfate proteoglycan (NG2)-positive (5).
Product Categories/Family for anti-S100B antibody
References
1. Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68. 2. Donato, R. (2003) Microsc Res Tech 60, 540-51. 3. Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22. 4. Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14. 5. Hachem, S. et al. (2005) Glia 51, 81-97.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11kDa
NCBI Official Full Name
protein S100-B
NCBI Official Synonym Full Names
S100 calcium binding protein B
NCBI Official Symbol
S100B
NCBI Official Synonym Symbols
NEF; S100; S100-B; S100beta
NCBI Protein Information
protein S100-B
UniProt Protein Name
Protein S100-B
UniProt Gene Name
S100B
Similar Products
Product Notes
The S100B s100b (Catalog #AAA266697) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S100B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the S100B s100b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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