Rabbit anti-Mouse CD40 Antibody | anti-CD40 antibody

Anti-CD40 [FGK45], Rabbit IgG

Cat. Num. AAA72268

• Gene Names: Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• Reactivity: Mouse
• Applications: Functional Assay, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: CD40; N/A; Anti-CD40 [FGK45], Rabbit IgG; Anti-CD40 [FGK45]; Tnfrsf5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; FGK4.5; anti-CD40 antibody

• Host: Rabbit
• Reactivity: Mouse
• Isotype: Rabbit IgG, Kappa
• Clone Number: FGK45
• Specificity: This antibody recognizes CD40. It is a type I transmembrane glycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD40 is expressed broadly on antigen-presenting cells (APCs) such as dendritic cells, B cells, macrophages, and monocytes as well as non-immune endothelial cells, basal epithelial cells, and a range of tumors. Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD40 antibody: FA (Functional Assay), WB (Western Blot), FCM/FACS (Flow Cytometry), Activity Assay, Antagonistic Antibody
• Immunogen: The original rat IgG2a antibody was raised against the recombinant mouse CD40 fusion protein.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometry using the Anti-CD40 antibody FGK45 (AAA72268). Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody or the rabbit IgG version of FGK45 (AAA72268-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

WB (Western Blot)

(Western Blot using Anti-CD40 antibody FGK45 (AAA72268). Mouse lymph node tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FGK45 (AAA72268-23.0) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

IF (Immunofluorescence)

(Immunofluorescence staining of mouse splenocytes with anti-CD40 (AAA72268) FGK45 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of FGK45 (AAA72268-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72268-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-CD40 antibody

FGK45 is an agonistic antibody that has been shown to activate CD40 expressing antigen presenting cells (APCs) what might promote anti-tumor T cell responses. For instance, the rat IgG2a version of this antibody was used to promote maturation of APCs what improved the ability of the macrophages to fight a tumour (Luheshi et al., 2016; pmid: 26918344). Another group used the same antibody to stimulate T-cells in a study on a tumor vaccine (Bartkowiak et al., 2015; pmid: 26351680). Others used ths clone as well to induce potent anti-tumor activity (Carmi et al., 2015; pmid: 25924063). On the other hand, it can also be used to inhibit CD40/CD154 interaction in vitro and in vivo. Furthermore, this clone can be also utilized to identify CD40-positive cells by flow cytometry (Rolink et al., 1996; pmid: 8885865). Administering this antibody can be also utilized to induce inflamatory bowel disease-like characteristics and create a mouse IBD model to study this class of diseases (Bauche et al., 2018; pmid: 30097293).

References

Rolink et al. The SCID but not the RAG-2 gene product is required for S mu-S epsilon heavy chain class switching Immunity. 1996 Oct;5(4):319-30. doi: 10.1016/s1074-7613(00)80258-7. PMID:8885865

NCBI and Uniprot Product Information

• NCBI GI #: 172046819
• NCBI GeneID: 21939
• UniProt Accession #: P27512; Q3TS33; Q3TSL2; Q3U799; Q3U7C9; Q3UBH3; Q542B1; Q8K2X6; Q99NE0; Q99NE1; Q99NE2; Q99NE3
• Molecular Weight: 28,878 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 5
• NCBI Official Synonym Full Names: CD40 antigen
• NCBI Official Symbol: Cd40
• NCBI Official Synonym Symbols: IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5; CD40L receptor; B-cell surface antigen CD40; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• UniProt Gene Name: Cd40
• UniProt Synonym Gene Names: Tnfrsf5
• UniProt Entry Name: TNR5_MOUSE