Chicken Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit | RANKL elisa kit

Chicken Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit

Cat. Num. AAA92145

• Gene Names: TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
• Reactivity: Chicken
• Synonyms: Receptor Activator of Nuclear Factor Kb Ligand; N/A; Chicken Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit; RANKL elisa kit

• Reactivity: Chicken
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.

• Intended Uses: This RANKL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken RANKL. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for RANKL elisa kit

Principle of the Assay: RANKL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RANKL antibody and an RANKL-HRP conjugate. The assay sample and buffer are incubated together with RANKL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RANKL concentration since RANKL from samples and RANKL-HRP conjugate compete for the anti-RANKL antibody binding site. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RANKL elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 2612922
• NCBI GeneID: 8600
• UniProt Accession #: O14788; O14723; Q96Q17; Q9P2Q3; Q54A98; Q5T9Y4
• Molecular Weight: 35,478 Da
• NCBI Official Full Name: RANKL
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: TNFSF11
• NCBI Official Synonym Symbols: ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: TNFSF11
• UniProt Synonym Gene Names: OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_HUMAN