Standard Curve (Sample)
Sheep Rap Guanine Nucleotide Exchange Factor 1 ELISA Kit | RAPGEF1 elisa kit
Sheep Rap Guanine Nucleotide Exchange Factor 1 ELISA Kit
Gene Names
RAPGEF1; C3G; GRF2
Reactivity
Sheep
Synonyms
Rap Guanine Nucleotide Exchange Factor 1; N/A; Sheep Rap Guanine Nucleotide Exchange Factor 1 ELISA Kit; RAPGEF1 elisa kit
Reactivity
Sheep
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for RAPGEF1 elisa kit
Principle of the assay: RAPGEF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAPGEF1 antibody and an RAPGEF1-HRP conjugate. The assay sample and buffer are incubated together with RAPGEF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAPGEF1 concentration since RAPGEF1 from samples and RAPGEF1-HRP conjugate compete for the anti-RAPGEF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RAPGEF1 from the sample, fewer sites are left to bind RAPGEF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAPGEF1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,735 Da
NCBI Official Full Name
rap guanine nucleotide exchange factor 1 isoform a
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 1
NCBI Official Symbol
RAPGEF1
NCBI Official Synonym Symbols
C3G; GRF2
NCBI Protein Information
rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
UniProt Protein Name
Rap guanine nucleotide exchange factor 1
UniProt Gene Name
RAPGEF1
UniProt Synonym Gene Names
GRF2
UniProt Entry Name
RPGF1_HUMAN
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Product Notes
The Sheep RAPGEF1 rapgef1 (Catalog #AAA96092) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA96092 ELISA Kit recognizes Sheep RAPGEF1. It is sometimes possible for the material contained within the vial of "Rap Guanine Nucleotide Exchange Factor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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