IF (Immunofluorescence)
(Immunofluorescence staining of rat splenocytes with anti-CD4 antibody OX-38 . Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of OX-38 (AAA72342) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72342, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Rat CD4 Antibody | anti-CD4 antibody
Anti-CD4 [OX-38]
Gene Names
Cd4; p55; W3/25
Reactivity
Rat
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Purified. Protein A affinity purified
Synonyms
CD4; N/A; Anti-CD4 [OX-38]; W3/25 antigen; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3;; anti-CD4 antibody
Reactivity
Rat
Isotype
Rabbit IgG kappa
Clone Number
OX-38
Specificity
This antibody binds rat CD4 cell surface antigen. CD4 is a transmembrane glycoprotein expressed on T helper cells, thymocytes, monocytes and macrophages. It plays an important role in the response of T helper cells to antigen. This antibody binds an identical or adjacent epitope to that bound by W3/25 antibody, and competes with mouse anti-rat CD4 antibody W3/25 for binding rat CD4.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD4 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of rat splenocytes with anti-CD4 antibody OX-38 . Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of OX-38 (AAA72342) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72342, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of rat splenocytes with anti-CD4 antibody OX-38 . Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of OX-38 (AAA72342) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72342, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-CD51 antibody NKI-M9 . HeLa cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of NKI-M9 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-CD51 antibody NKI-M9 . HeLa cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of NKI-M9 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-CD4 antibody
The original antibody was generated by immunizing mice with rat lymphocytes or rat thymocyte glycoproteins.
Describes the generation of this antibody and its use in the characterization of the CD4 (W3/25) antigen present on rat peritoneal macrophages.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Describes the generation of this antibody and its use in the characterization of the CD4 (W3/25) antigen present on rat peritoneal macrophages.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-CD4 antibody
References
Jefferies et al.
J Exp Med. 1985 Jul 1;162(1):117-27.
J Exp Med. 1985 Jul 1;162(1):117-27.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,438 Da
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Synonym Full Names
Cd4 molecule
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
p55; W3/25
NCBI Protein Information
T-cell surface glycoprotein CD4; CD4 antigen; T-cell surface antigen T4/Leu-3; W3/25 antigen
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_RAT
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Product Notes
The CD4 cd4 (Catalog #AAA72342) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD4 [OX-38] reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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