IF (Immunofluorescence)
(Immunofluorescence staining of C6 cells with anti-NMDA Receptor 17 Immunofluorescence analysis of paraformaldehyde fixed C6 cells stained with the chimeric rabbit IgG version of 17 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Rat NMDA Receptor Antibody | anti-NMDA antibody
Anti-NMDA Receptor [17]
Gene Names
Grin2b; GluN2B
Reactivity
Rat
Applications
Crystallography
Purity
Purified. Protein A affinity purified
Synonyms
NMDA Receptor; N/A; Anti-NMDA Receptor [17]; NMDAR; NMDA-R; Glutamate receptor; anti-NMDA antibody
Reactivity
Rat
Isotype
Mouse IgG1 kappa
Clone Number
17
Specificity
Reacts specifically with rat GluN2B ATD.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NMDA antibody
Crystallography
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of C6 cells with anti-NMDA Receptor 17 Immunofluorescence analysis of paraformaldehyde fixed C6 cells stained with the chimeric rabbit IgG version of 17 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of C6 cells with anti-NMDA Receptor 17 Immunofluorescence analysis of paraformaldehyde fixed C6 cells stained with the chimeric rabbit IgG version of 17 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of C6 cells with anti-NMDA Receptor (isotype control, black line) or the rabbit IgG version of 17 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of C6 cells with anti-NMDA Receptor (isotype control, black line) or the rabbit IgG version of 17 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-NMDA antibody
Monoclonal antibodies that bind rat GluN2B ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis followed by re-chromatographing onto Protein-A Sepharose to remove Fc.
Describes the lack of high resolution structures representing the active state of the NMDA receptor.
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Describes the lack of high resolution structures representing the active state of the NMDA receptor.
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for anti-NMDA antibody
References
Tajima et al.
Nature. 2016 May 2; 534(7605): 63-68
Nature. 2016 May 2; 534(7605): 63-68
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
166,071 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol
Grin2b
NCBI Official Synonym Symbols
GluN2B
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, NMDA2B
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Gene Name
Grin2b
UniProt Synonym Gene Names
GluN2B; NMDAR2B; NR2B
UniProt Entry Name
NMDE2_RAT
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Product Notes
The NMDA grin2b (Catalog #AAA72301) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-NMDA Receptor [17] reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NMDA Receptor can be used in a range of immunoassay formats including, but not limited to, Crystallography. Researchers should empirically determine the suitability of the NMDA grin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMDA Receptor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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