Standard Curve (Sample)
(Typical Standard Curve for ALPL CLIA (Sandwich))
Rat Alkaline Phosphatase, Tissue-nonspecific (ALPL) CLIA Kit | ALPL clia kit
Rat Alkaline Phosphatase, Tissue-nonspecific (ALPL) CLIA Kit
Gene Names
Alpl; Akp2; PHOA
Reactivity
Rat
Synonyms
Alkaline Phosphatase, Tissue-nonspecific (ALPL); N/A; Rat Alkaline Phosphatase, Tissue-nonspecific (ALPL) CLIA Kit; ALPL CLIA kit (Rat); BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase, Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney; ALPL clia kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Tissue-nonspecific (ALPL). No significant cross-reactivity or interference between Alkaline Phosphatase, Tissue-nonspecific (ALPL) and analogues was observed.
Reactive with Rat ALPL/Alkaline Phosphatase, Tissue-nonspecific
Reactive with Rat ALPL/Alkaline Phosphatase, Tissue-nonspecific
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Method
Chemiluminescence
Featured Series
SE kit
Detection Range
0.27-200ng/mL
Sensitivity
0.12ng/mL
Sample Size
100uL
Assay Time
2h, 40min
Function
Detects protein (regular version)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Tissue-nonspecific (ALPL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Tissue-nonspecific (ALPL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Tissue-nonspecific (ALPL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Tissue-nonspecific (ALPL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Tissue-nonspecific (ALPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alkaline Phosphatase, Tissue-nonspecific (ALPL) level in the sample or standard.
Research Area
Enzyme & Kinase, Metabolic pathway, Hepatology, Bone metabolism
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
(Typical Standard Curve for ALPL CLIA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for ALPL CLIA (Sandwich))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,659 Da
NCBI Official Full Name
Alkaline phosphatase, tissue-nonspecific isozyme
NCBI Official Synonym Full Names
alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol
Alpl
NCBI Official Synonym Symbols
Akp2; PHOA
NCBI Protein Information
alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; AP-TNAP; alkaline phosphatase 2, liver; tissue-nonspecific alkaline phosphatase; Tissue-nonspecific ALP alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
UniProt Protein Name
Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Gene Name
Alpl
UniProt Synonym Gene Names
AP-TNAP; TNSALP
UniProt Entry Name
PPBT_RAT
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Product Notes
The Rat ALPL alpl (Catalog #AAA161514) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161514 CLIA Kit recognizes Rat ALPL. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Tissue-nonspecific (ALPL), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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