Standard Curve (Sample)
Rat Growth Hormone (GH) CLIA Kit | GH clia kit
Rat Growth Hormone (GH) CLIA Kit
Gene Names
Gh1; Gh; RNGHGP
Reactivity
Rat
Applications
CLIA (Chemiluminescence Immunoassay)
Synonyms
Growth Hormone (GH); N/A; Rat Growth Hormone (GH) CLIA Kit; GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal; GH clia kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of growth hormone. No significant cross-reactivity or interference between growth hormone and analogues was observed.
Assay Type
Competitive Inhibition
Samples
Serum, Plasma and other biological fluids
Detection Range
16-10,000pg/mL
Sensitivity
5pg/mL
Applicable Applications for GH clia kit
CLIA (Chemiluminescence Immunoassay)
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level growth hormone were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level growth hormone were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GH clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of growth hormone in rat serum, plasma and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to growth hormone. A competitive inhibition reaction is launched between biotin labeled growth hormone and unlabeled growth hormone (Standards or samples) with the pre-coated antibody specific to growth hormone. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of growth hormone in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the growth hormone level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to growth hormone. A competitive inhibition reaction is launched between biotin labeled growth hormone and unlabeled growth hormone (Standards or samples) with the pre-coated antibody specific to growth hormone. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of growth hormone in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the growth hormone level in the sample or standard.
Product Categories/Family for GH clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,656 Da
NCBI Official Full Name
somatotropin
NCBI Official Synonym Full Names
growth hormone 1
NCBI Official Symbol
Gh1
NCBI Official Synonym Symbols
Gh; RNGHGP
NCBI Protein Information
somatotropin
UniProt Protein Name
Somatotropin
UniProt Gene Name
Gh1
UniProt Synonym Gene Names
Gh
UniProt Entry Name
SOMA_RAT
Similar Products
Product Notes
The Rat GH gh1 (Catalog #AAA150366) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA150366 CLIA Kit recognizes Rat GH. AAA Biotech's Growth Hormone (GH) can be used in a range of immunoassay formats including, but not limited to, CLIA (Chemiluminescence Immunoassay). Researchers should empirically determine the suitability of the GH gh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Growth Hormone (GH), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.