Rat RANTES CLIA Kit | RANTES clia kit

RANTES (Rat) LumiAb ELISA Kit

Cat. Num. AAA318588

• Gene Names: Ccl5; Scya5; Rantes
• Reactivity: Rat
• Synonyms: RANTES; N/A; RANTES (Rat) LumiAb ELISA Kit; Rat RANTES; C-C motif chemLumine 5; SIS-delta; Small-inducible cytLumine A5; T-cell-specific protein RANTES; Scya5; RANTES clia kit

• Reactivity: Rat
• Specificity: The LumiAb Rat MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Rat MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 92
• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for RANTES clia kit

Principle of the Assay: The LumiAb Rat MCP-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Rat MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat MCP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Rat MCP-1 or Monocyte Chemotactic Protein 1, also known as C-C Motif Chemokine 2, is a 148 amino acid cytokine protein encoded by the Ccl2 gene located at locus 10q26 on chromosome 10. After initial synthesis, the 23 residue signal sequence is cleaved from the N-terminal region, allowing for proper folding and maturation of the MCP-1 peptide. MCP-1, a monomeric or homodimeric intercrine beta (chemokine C-C) family member is known to be a chemotactic factor that attracts monocytes but not neutrophils. Structurally, it consists of 2 disulfide linkages and a possible glycosylation site at residue 126.

Product Categories/Family for RANTES clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 1711393
• NCBI GeneID: 81780
• UniProt Accession #: P50231
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: Scya5; Rantes
• NCBI Protein Information: C-C motif chemokine 5
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_RAT