Standard Curve (Sample)
Rat Vasoactive Intestinal Peptide (VIP) DIY ELISA Kit | VIP diy elisa kit
Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit DIY Materials
Gene Names
VIP; PHM27
Reactivity
Rat
Synonyms
Vasoactive Intestinal Peptide (VIP); N/A; Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit DIY Materials; PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27; VIP diy elisa kit
Reactivity
Rat
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP). No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.
Sequence Length
169
Assay Type
Competitive Inhibition ELISA for Antigen Detection
Samples
Serum, plasma and other biological fluids.
Detection Range
6.17-500pg/mL
Sensitivity
2.58pg/mL.
Reagent Contents
Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 2. ( )
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Product Categories/Family for VIP diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,082 Da
NCBI Official Full Name
VIP peptides isoform 2 preproprotein
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
VIP
NCBI Official Synonym Symbols
PHM27
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
UniProt Gene Name
VIP
UniProt Synonym Gene Names
VIP
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Product Notes
The Rat VIP vip (Catalog #AAA147594) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA147594 DIY ELISA Kit recognizes Rat VIP. It is sometimes possible for the material contained within the vial of "Vasoactive Intestinal Peptide (VIP), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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