Standard Curve (Sample)
Rat 17-beta-hydroxysteroid dehydrogenase 14 (HSD17B14) ELISA Kit | HSD17B14 elisa kit
Rat 17-beta-hydroxysteroid dehydrogenase 14 (HSD17B14) ELISA Kit
Gene Names
HSD17B14; DHRS10; SDR47C1; retSDR3
Reactivity
Rat
Synonyms
17-beta-hydroxysteroid dehydrogenase 14 (HSD17B14); N/A; Rat 17-beta-hydroxysteroid dehydrogenase 14 (HSD17B14) ELISA Kit; HSD17B14 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of HSD17B14. No significant cross-reactivity or interference between HSD17B14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSD17B14 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HSD17B14 elisa kit
Principle of the Assay: HSD17B14 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSD17B14 antibody and an HSD17B14-HRP conjugate. The assay sample and buffer are incubated together with HSD17B14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSD17B14 concentration since HSD17B14 from samples and HSD17B14-HRP conjugate compete for the anti-HSD17B14 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSD17B14 from the sample, fewer sites are left to bind HSD17B14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSD17B14 concentration in each sample is interpolated from this standard curve.
Intended Uses: This HSD17B14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HSD17B14. This ELISA kit for research use only, not for therapeutic or test applications!
Intended Uses: This HSD17B14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HSD17B14. This ELISA kit for research use only, not for therapeutic or test applications!
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,317 Da
NCBI Official Full Name
17-beta-hydroxysteroid dehydrogenase 14
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 14
NCBI Official Symbol
HSD17B14
NCBI Official Synonym Symbols
DHRS10; SDR47C1; retSDR3
NCBI Protein Information
17-beta-hydroxysteroid dehydrogenase 14; 17-beta-HSD 14; 17-beta-hydroxysteroid dehydrogenase DHRS10; dehydrogenase/reductase SDR family member 10; retinal short-chain dehydrogenase/reductase 3; retinal short-chain dehydrogenase/reductase retSDR3; short chain dehydrogenase/reductase family 47C, member 1
UniProt Protein Name
17-beta-hydroxysteroid dehydrogenase 14
UniProt Gene Name
HSD17B14
UniProt Synonym Gene Names
DHRS10; SDR3; UNQ502/PRO474; 17-beta-HSD 14
UniProt Entry Name
DHB14_HUMAN
Similar Products
Product Notes
The Rat HSD17B14 hsd17b14 (Catalog #AAA245986) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245986 ELISA Kit recognizes Rat HSD17B14. It is sometimes possible for the material contained within the vial of "17-beta-hydroxysteroid dehydrogenase 14 (HSD17B14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.