Standard Curve (Sample)
Rat ANG II ELISA Kit | ANG II elisa kit
Rat ANG II (Angiotensin II) ELISA Kit
Gene Names
Agt; Ang; PAT; ANRT; AngII
Reactivity
Rat
Synonyms
ANG II; Rat ANG II (Angiotensin II) ELISA Kit; ANG II elisa kit
Reactivity
Rat
Specificity
This kit recognizes Rat Ang- II in samples. No significant cross-reactivity or interference between Rat Ang- II and analogues was observed.
Sequence Length
477
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
15.63-1000pg/mL
Sensitivity
9.38pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Ang- II were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Ang- II were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ANG II elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat Ang- II concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang- II. During the reaction, Rat Ang- II in the sample or standard competes with a fixed amount of Rat Ang- II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang- II. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat Ang- II in the samples is then determined by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang- II. During the reaction, Rat Ang- II in the sample or standard competes with a fixed amount of Rat Ang- II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang- II. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat Ang- II in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,982 Da
NCBI Official Full Name
angiotensinogen
NCBI Official Synonym Full Names
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
NCBI Official Symbol
Agt
NCBI Official Synonym Symbols
Ang; PAT; ANRT; AngII
NCBI Protein Information
angiotensinogen; angiotensin II; angiotensinogen (PAT); serpin A8
UniProt Protein Name
Angiotensinogen
UniProt Gene Name
Agt
UniProt Synonym Gene Names
Serpina8; Ang I; Ang II; Ang III; Ang IV
UniProt Entry Name
ANGT_RAT
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Product Notes
The Rat ANG II agt (Catalog #AAA21867) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA21867 ELISA Kit recognizes Rat ANG II. It is sometimes possible for the material contained within the vial of "ANG II, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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