Standard Curve (Sample)
Rat Aquaporin-12A (AQP12A) ELISA Kit | AQP12A elisa kit
Rat Aquaporin-12A (AQP12A) ELISA Kit
Reactivity
Rat
Synonyms
Aquaporin-12A (AQP12A); N/A; Rat Aquaporin-12A (AQP12A) ELISA Kit; AQP12A elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of AQP12A. No significant cross-reactivity or interference between AQP12A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AQP12A and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
258
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AQP12A elisa kit
Principle of the Assay: AQP12A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AQP12A antibody and an AQP12A-HRP conjugate. The assay sample and buffer are incubated together with AQP12A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP12A concentration since AQP12A from samples and AQP12A-HRP conjugate compete for the anti-AQP12A antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP12A from the sample, fewer sites are left to bind AQP12A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP12A concentration in each sample is interpolated from this standard curve.
Intended Uses: This AQP12A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AQP12A. This ELISA kit for research use only, not for therapeutic or test applications!
Intended Uses: This AQP12A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AQP12A. This ELISA kit for research use only, not for therapeutic or test applications!
Product Categories/Family for AQP12A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
28,246 Da
NCBI Official Full Name
aquaporin-12A
UniProt Protein Name
Aquaporin-12A
Similar Products
Product Notes
The Rat AQP12A (Catalog #AAA247281) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA247281 ELISA Kit recognizes Rat AQP12A. It is sometimes possible for the material contained within the vial of "Aquaporin-12A (AQP12A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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