Standard Curve (Sample)
Rat Atrial Natriuretic Peptide (ANP) ELISA Kit | ANP elisa kit
Rat Atrial Natriuretic Peptide (ANP) Mini Samples ELISA Kit
Gene Names
Nppa; ANF; ANP; Pnd; RATANF
Reactivity
Rat
Synonyms
Atrial Natriuretic Peptide (ANP); N/A; Rat Atrial Natriuretic Peptide (ANP) Mini Samples ELISA Kit; ANH; ANF; PND; NPPA; CDD-ANF; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Natriuretic Peptide Precursor A; Prepronatriodilatin; Cardiodilatin-related peptide; ANP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of AngII. No significant cross-reactivity or interference between AngII and analogues was observed.
Sequence Length
152
Samples
Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
Assay Type
Quantitative Competitive
Detection Range
62.5-4000pg/mL
Sensitivity
24.31pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AngII were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AngII were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ANP elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to AngII has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AngII and unlabeled AngII (Standards or samples) with the pre-coated antibody specific to AngII. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AngII in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AngII in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to AngII has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AngII and unlabeled AngII (Standards or samples) with the pre-coated antibody specific to AngII. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AngII in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AngII in the sample.
Product Categories/Family for ANP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,556 Da
NCBI Official Full Name
natriuretic peptides A
NCBI Official Synonym Full Names
natriuretic peptide A
NCBI Official Symbol
Nppa
NCBI Official Synonym Symbols
ANF; ANP; Pnd; RATANF
NCBI Protein Information
natriuretic peptides A
UniProt Protein Name
Natriuretic peptides A
UniProt Gene Name
Nppa
UniProt Synonym Gene Names
ANF; ANP
Similar Products
Product Notes
The Rat ANP nppa (Catalog #AAA147330) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA147330 ELISA Kit recognizes Rat ANP. It is sometimes possible for the material contained within the vial of "Atrial Natriuretic Peptide (ANP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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