Rat CCL4/MIP-1 beta ELISA Kit | CCL4 elisa kit

Rat CCL4/MIP-1 beta PicoKine ELISA Kit

Cat. Num. AAA45588

• Gene Names: Ccl4; Scya4; Mip1-b
• Reactivity: Rat
• Synonyms: CCL4/MIP-1 beta; N/A; Rat CCL4/MIP-1 beta PicoKine ELISA Kit; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; ACT-2; ACT2; AT744.1; CC chemokine ligand 4; CCL4; CCL4_HUMAN; G-26 T-lymphocyte-secreted protein; HC21; LAG-1; LAG1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP-1-beta; MIP-1-beta(1-69); MIP-1-beta(3-69); MIP1-B; MIP1B; MIP1B1; PAT 744; Protein H400; SCYA2; SCYA4; SIS-gamma; Small-inducible cytokine A4; T-cell activation protein 2; CCL4 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat CCL4
• Sequence Length: 92
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Rat CCL4/MIP-1 beta PicoKine ELISA Kit standard curve)

Related Product Information for CCL4 elisa kit

Principle of the assay: rat CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.Coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CCL4 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).

NCBI and Uniprot Product Information

• NCBI GI #: 1709025
• NCBI GeneID: 116637
• UniProt Accession #: P50230; A1EC89
• Molecular Weight: 10,234 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: Ccl4
• NCBI Official Synonym Symbols: Scya4; Mip1-b
• NCBI Protein Information: C-C motif chemokine 4; MIP-1-beta; small inducible cytokine A4; small-inducible cytokine A4; macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1 beta
• UniProt Protein Name: C-C motif chemokine 4
• UniProt Gene Name: Ccl4
• UniProt Synonym Gene Names: Mip1b; Scya4; MIP-1-beta
• UniProt Entry Name: CCL4_RAT