Rat CCL4/MIP1beta ELISA Kit | CCL4/MIP1beta elisa kit

Rat CCL4/MIP1beta PicoKine Quick ELISA Kit

Cat. Num. AAA128080

• Gene Names: Ccl4; Scya4; Mip1-b
• Reactivity: Rat
• Applications: ELISA
• Synonyms: CCL4/MIP1beta; N/A; Rat CCL4/MIP1beta PicoKine Quick ELISA Kit; C-C motif chemokine 4; Macrophage inflammatory protein 1-beta; MIP-1-beta; Small-inducible cytokine A4; Ccl4; Mip1b, Scya4; CCL4/MIP1beta elisa kit

• Reactivity: Rat
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Assay Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: A24-N92
• Anticoagulant: heparin, EDTA or citrate
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CCL4/MIP1beta elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.018	0.096	0.162	0.336	0.636	1.267	1.691	2.040

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	31	144	453	31	148	419
  Standard deviation	1.42	9.21	24.31	1.67	12.13	24.3
  CV (%)	4.6%	6.4%	5.5%	5.4%	8.2%	5.8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	31	27	31	27	29	2	6.8%
  Sample 2	144	160	147	149	150	6.04	4%
  Sample 3	453	458	508	520	484	29.6	6.1%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Rat CCL4/MIP-1 beta PicoKine ELISA Kit standard curve)

Related Product Information for CCL4/MIP1beta elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CCL4/MIP1beta elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 25742843
• NCBI GeneID: 116637
• NCBI Accession #: NP_446310.1
• NCBI GenBank Nucleotide #: NM_053858.1
• UniProt Accession #: P50230; A1EC89
• Molecular Weight: 10,234 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: Ccl4
• NCBI Official Synonym Symbols: Scya4; Mip1-b
• NCBI Protein Information: C-C motif chemokine 4; MIP-1-beta; macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1 beta; small inducible cytokine A4; small-inducible cytokine A4
• UniProt Protein Name: C-C motif chemokine 4
• UniProt Gene Name: Ccl4
• UniProt Synonym Gene Names: Mip1b; Scya4; MIP-1-beta
• UniProt Entry Name: CCL4_RAT