Rat CD25/IL2RA ELISA Kit | CD25/IL2RA elisa kit

Rat CD25/IL2RA ELISA Kit PicoKine

Cat. Num. AAA128022

• Gene Names: Il2ra; IL2RAC
• Reactivity: Rat
• Applications: ELISA
• Synonyms: CD25/IL2RA; N/A; Rat CD25/IL2RA ELISA Kit PicoKine; CD25/IL2RA elisa kit

• Reactivity: Rat
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 93.7pg/ml-6,000pg/ml
• Sensitivity: <45pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Immunogen: Expression system for standard: CHO; Immunogen sequence: E22-Q235
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CD25/IL2RA elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	93.7	187.5	375	750	1,500	3,000	6,000
  O.D.	0.019	0.084	0.156	0.395	0.581	0.881	1.275	1.831

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	114	306	2233	155	316	2793
  Standard deviation	6.68	15.67	64.09	10.37	22.03	161.99
  CV(%)	5.9%	5.1%	2.9%	6.7%	7%	5.8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	114	154	167	139	144	4.69	3.3%
  Sample 2	306	243	257	203	252	19.55	7.8%
  Sample 3	2233	2818	2456	2488	2499	151.67	6.1%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Rat CD25 PicoKine ELISA Kit standard curve)

Related Product Information for CD25/IL2RA elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CD25/IL2RA elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 7710038
• NCBI GeneID: 25704
• NCBI Accession #: NP_037295.1
• NCBI GenBank Nucleotide #: NM_013163.1
• UniProt Accession #: P26897
• Molecular Weight: 30,557 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha
• NCBI Official Symbol: Il2ra
• NCBI Official Synonym Symbols: IL2RAC
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL-2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha chain
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: Il2ra
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_RAT