Standard Curve (Sample)
(Rat CD86/B7-2 PicoKine ELISA Kit standard curve)
Rat CD86/B7-2 ELISA Kit | CD86 elisa kit
Rat CD86/B7-2 PicoKine ELISA Kit
Gene Names
Cd86; B7-2
Reactivity
Rat
Synonyms
CD86/B7-2; N/A; Rat CD86/B7-2 PicoKine ELISA Kit; T-lymphocyte activation antigen CD86; CD86 molecule; Activation B7 2 antigen; B7 2; B70; B72; BU63; CD28LG2; CD86 antigen (CD28 antigen ligand 2 B7 2 antigen); CTLA 4 counter receptor B7.2; FUN 1; FUN1; T lymphocyte activation antigen CD86 precursor; CD86 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat CD86
Sequence Length
149
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Rat CD86/B7-2 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Rat CD86/B7-2 PicoKine ELISA Kit standard curve)
Related Product Information for CD86 elisa kit
Principle of the assay: Rat CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD86 has been precoated onto 96-well plates. Standards(NSO, V29-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD86 amount of sample captured in plate.
Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
17,153 Da
NCBI Official Full Name
B7-2
NCBI Official Synonym Full Names
CD86 molecule
NCBI Official Symbol
Cd86
NCBI Official Synonym Symbols
B7-2
NCBI Protein Information
T-lymphocyte activation antigen CD86; cd86 antigen; membrane glycoprotein
UniProt Protein Name
B7-2
UniProt Gene Name
Cd86
UniProt Entry Name
Q62810_RAT
Similar Products
Product Notes
The Rat CD86 cd86 (Catalog #AAA45528) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45528 ELISA Kit recognizes Rat CD86. It is sometimes possible for the material contained within the vial of "CD86/B7-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.