Rat Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit | F13A1 elisa kit

Rat Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit

Cat. Num. AAA66407

• Gene Names: F13a1; F13a
• Reactivity: Rat
• Synonyms: Coagulation Factor XIII A1 Polypeptide (F13A1); N/A; Rat Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit; F13-A1; FXIIIA1; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain; Coagulation factor XIII A chain; F13A1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of F13A1. No significant cross-reactivity or interference between F13A1 and analogues was observed.
• Sequence Length: 732
• Samples: Plasma.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.56ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F13A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F13A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for F13A1 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of F13A1 in rat plasma.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F13A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F13A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F13A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F13A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 158186711
• NCBI GeneID: 60327
• NCBI Accession #: NP_067730.2
• NCBI GenBank Nucleotide #: NM_021698.2
• UniProt Accession #: O08619
• Molecular Weight: 82,659 Da
• NCBI Official Full Name: coagulation factor XIII A chain
• NCBI Official Synonym Full Names: coagulation factor XIII A1 chain
• NCBI Official Symbol: F13a1
• NCBI Official Synonym Symbols: F13a
• NCBI Protein Information: coagulation factor XIII A chain
• UniProt Protein Name: Coagulation factor XIII A chain
• UniProt Gene Name: F13a1
• UniProt Synonym Gene Names: F13a; Coagulation factor XIIIa