Rat Collagen Type I (COL1) ELISA Kit | COL1 elisa kit

Rat Collagen Type I (COL1) ELISA Kit

Cat. Num. AAA20716

• Gene Names: COL1A2; OI4
• Reactivity: Rat
• Synonyms: Collagen Type I (COL1); N/A; Rat Collagen Type I (COL1) ELISA Kit; COL1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL1. No significant cross-reactivity or interference between COL1 and analogues was observed.
• Sequence Length: 1,366
• Assay Type: Quantitative Sandwich
• Samples: Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.219ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for COL1 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for COL1 elisa kit

Metabolic pathway; Developmental science; Bone metabolism

References

Temporal changes in the muscle extracellular matrix due to volumetric muscle loss injury; Connect Tissue Res; 2022 Mar;63(2):124-137.; PMID: 33535825; Daniel B Hoffman; School of Kinesiology, University of Minnesota, Minneapolis, MN, USA.

NCBI and Uniprot Product Information

• NCBI GI #: 45708783
• NCBI GeneID: 1278
• Molecular Weight: 129,314 Da
• NCBI Official Full Name: Collagen, type I, alpha 2
• NCBI Official Synonym Full Names: collagen, type I, alpha 2
• NCBI Official Symbol: COL1A2
• NCBI Official Synonym Symbols: OI4
• NCBI Protein Information: collagen alpha-2(I) chain
• UniProt Protein Name: Collagen alpha-2(I) chain
• UniProt Gene Name: COL1A2
• UniProt Entry Name: CO1A2_HUMAN