Rat Crossveinless-2/CV-2/BMPER ELISA Kit | Bmper elisa kit

Rat Crossveinless-2/CV-2/BMPER ELISA Kit

Cat. Num. AAA125841

• Reactivity: Rat
• Synonyms: Crossveinless-2/CV-2/BMPER; N/A; Rat Crossveinless-2/CV-2/BMPER ELISA Kit; CV2; CV-2; CRIM3; BMP-binding endothelial regulator; Bmper elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat BMPER
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5pg/ml-4,000pg/ml
• Sensitivity: <50pg/ml
• Capture/Detection Antibodies: Capture antibody- a polyclonal antibody from goat|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.068|0.172|0.254|0.376|0.612|0.922|1.624|2.289
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|226|813|1749|; Standard deviation|10.62|45.53|101.44|; CV(%)|4.7%|5.6%|5.8%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|235|854|1806; Standard deviation|15.04|62.34|140.87; CV(%)|6.4%|7.3%|7.8%

• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 226|217|208|243|224|10.53|4.7%; Sample 2: 813|835|829|846|831|49.03|5.9%; Sample 3: 1749|1876|1816|1721|1791|110.98|6.2%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: V34-R685
• Anticoagulant: heparin, EDTA or citrate
• Principle: Rat BMPER ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for BMPER has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: V34-R685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMPER is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMPER amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml rat BMPER standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. We recommended that each rat BMPER standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Application Data

Related Product Information for Bmper elisa kit

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.

Product Categories/Family for Bmper elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GeneID: 300455
• UniProt Accession #: F1M661