Standard Curve (Sample)
Rat Cytochrome c oxidase subunit 8B, mitochondrial (COX8B) ELISA Kit | COX8B elisa kit
Rat Cytochrome c oxidase subunit 8B, mitochondrial (COX8B) ELISA Kit
Gene Names
Cox8b; CCO8A; Cox8h; COXVIII-H
Reactivity
Rat
Synonyms
Cytochrome c oxidase subunit 8B, mitochondrial (COX8B); N/A; Rat Cytochrome c oxidase subunit 8B, mitochondrial (COX8B) ELISA Kit; COX8B elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for COX8B elisa kit
Intended Uses: This COX8B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat COX8B. This ELISA kit for research use only!
Principle of the Assay||COX8B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX8B antibody and an COX8B-HRP conjugate. The assay sample and buffer are incubated together with COX8B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX8B concentration since COX8B from samples and COX8B-HRP conjugate compete for the anti-COX8B antibody binding site. Since the number of sites is limited, as more sites are occupied by COX8B from the sample, fewer sites are left to bind COX8B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX8B concentration in each sample is interpolated from this standard curve.
Principle of the Assay||COX8B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX8B antibody and an COX8B-HRP conjugate. The assay sample and buffer are incubated together with COX8B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX8B concentration since COX8B from samples and COX8B-HRP conjugate compete for the anti-COX8B antibody binding site. Since the number of sites is limited, as more sites are occupied by COX8B from the sample, fewer sites are left to bind COX8B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX8B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,549 Da
NCBI Official Full Name
cytochrome c oxidase subunit 8B, mitochondrial
NCBI Official Synonym Full Names
cytochrome c oxidase, subunit VIIIb
NCBI Official Symbol
Cox8b
NCBI Official Synonym Symbols
CCO8A; Cox8h; COXVIII-H
NCBI Protein Information
cytochrome c oxidase subunit 8B, mitochondrial; cytochrome c oxidase subunit 8H; cytochrome c oxidase subunit 8-1; cytochrome c oxidase, subunit 8B; cytochrome c oxidase polypeptide VIII-heart; cytochrome c oxidase, subunit VIIIb, muscle; Cytochrom c oxidase subunit VIII-H (heart/muscle); Cytochrome c oxidase subunit VIII-H (heart/muscle)
UniProt Protein Name
Cytochrome c oxidase subunit 8B, mitochondrial
UniProt Gene Name
Cox8b
UniProt Synonym Gene Names
Cox8h
UniProt Entry Name
COX8B_RAT
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Product Notes
The Rat COX8B cox8b (Catalog #AAA246170) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246170 ELISA Kit recognizes Rat COX8B. It is sometimes possible for the material contained within the vial of "Cytochrome c oxidase subunit 8B, mitochondrial (COX8B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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